技術
HPLC: suitable
測試參數
column: Ascentis Express RP-Amide, 3 cm x 4.6 mm I.D., 2.7 μm particles (53921-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.6 with acetic acid; [B] water; [C] acetonitrile
gradient: 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
flow rate: 3 mL/min
pressure: 3336 psi (230 bar)
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 mL/min
sample: diet soda spiked with neotame 350 μg/mL
適合性
application for HPLC
應用
food and beverages
分析報告
This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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