技術
HPLC: suitable
測試參數
column: Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.6 (titrated with acetic acid); [B] water; [C] acetonitrile
gradient: 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
flow rate: 3 mL/min
pressure: 3191 psi (220 bar)
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: neotame 350 μg/mL in diet cola
適合性
application for HPLC
應用
food and beverages
分析報告
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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