G003763

HPLC Analysis of Cortocisteroids in Urine on Discovery^® HS F5 after SPE using Discovery^® DSC-CN

application for SPE, application for HPLC

属性

• 技術: HPLC: suitable; solid phase extraction (SPE): suitable
• 測試參數: sample preparation: SPE (Solid Phase Extraction); sample/matrix: human urine spiked with corticosteroids at 0.5 and 1.0 μg/mL and diluted in DI water (1:1, v/v); SPE well plate: Discovery DSC-CN 96-well SPE, 100 mg/well (575636-U); condition: 1 mL methanol; 1 mL DI water.; sample addition: 1 mL spiked urine; washing: 1 mL 20% methanol; elution: 1 mL methanol; eluate post-treatment: Evaporate eluate with nitrogen at 30 °C. Reconstitute in 200 μL mobile phase.; column: Discovery HS F5, 5 cm x 4.6 mm I.D., 3 μm particles (567504-U); mobile phase: [A] methanol; [B] DI water; (40:60, A:B); flow rate: 1.5 mL/min; column temp.: 35 °C; detector: UV, 240 nm; injection: 5 μL
• 適合性: application for HPLC; application for SPE
• 應用: clinical

说明

法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany