技術
HPLC: suitable
測試參數
column: Discovery BIO Wide Pore C18, 15 cm × 2.1 mm I.D., 3 μm particles (568222-U)
mobile phase: [A] 0.1% TFA in water; [B] 0.1% TFA in water:0.1% TFA in acetonitrile, 50:50;
gradient: 0-100% B in 50 min
flow rate: 0.2 mL/min
column temp.: 35°C
detector: UV, 220 nm
injection: 5 μL, each at 0.8 mg/mL in 0.1% TFA
適合性
application for HPLC
應用
life science and biopharma
法律資訊
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Apohemoglobin, carboxymethylated, tryptic digest
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