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材料
stainless steel
agency
suitable for USP L1
產品線
TSKgel®
製造商/商標名
Tosoh ODS-80Tm, 08149
標籤範圍
15% Carbon, monolayer, endcapped
參數
0-100% organic solvent
0-45 °C temperature
1.2 mL/min flow rate
310 bar max. pressure
技術
HPLC: suitable
長度 × 內徑
25 cm × 4.6 mm
表面積
425 m2/g
基質
silica particle platform
基質活性組
C18 (octadecyl) phase
粒徑
5 μm
孔徑
100 Å pore diameter
工作pH值
2.0-7.5
分離技術
reversed phase
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一般說明
TSKgel ODS-80Tm is a general purpose column for the analysis of low MW pharmaceuticals, bases, nucleosides and nucleotides. Common applications include purity checks and peptide mapping. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-80Tm has 80Å nominal pore size. The pore size of the base silica is 100Å.
應用
TSKgel® ODS-80Tm HPLC Column may be used for the separation of:
- Dehydroacetic acid, benzoic acid, sorbic acid and salicylic acid in cosmetic products during their determination by solid-phase extraction (SPE) followed by analysis using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection.
- Cilostazol and its metabolites in human urine during their determination by liquid-liquid extraction (LLE) followed by analysis using HPLC-UV.
- Heterocyclic amines in food products during their analysis by LC coupled to mass spectrometry with electrospray ionization (ESI-MS), LC combined with tandem mass spectrometry (MS/MS) with ESI, LC coupled to atmospheric pressure chemical ionization (APCI)-MS/MS, LC with UV-diode array detection (DAD), LC with UV-fluorescence detection (FLD) and LC in conjunction with electrochemical detection, etc.
- Rabeprazole and its metabolite, rabeprazole thioether in human plasma samples during their analysis by column switching HPLC with UV detection.
- β-lactam antibiotic, cefaclor during its determination by HPLC-FLD.
外觀
Shipped in 70% methanol - 30% water.
法律資訊
TSKgel is a registered trademark of Tosoh Corporation
Sensitive determination of a β-lactam antibiotic, cefaclor by liquid chromatography with chemiluminescence detection.
Journal of Pharmaceutical and Biomedical Analysis, 30(6), 1765-1771 (2003)
Analysis of heterocyclic amines in food products: interlaboratory studies
Journal of Chromatography. B, Biomedical Sciences and Applications, 802(1), 69-78 (2004)
Determination of cilostazol and its metabolites in human urine by high performance liquid chromatography.
Journal of Pharmaceutical and Biomedical Analysis, 24(3), 381-389 (2001)
Determination of rabeprazole and its active metabolite, rabeprazole thioether in human plasma by column-switching high-performance liquid chromatography and its application to pharmacokinetic study.
Journal of Chromatography. B, Biomedical Sciences and Applications, 824(1-2), 238-243 (2005)
Journal of pharmaceutical and biomedical analysis, 28(2), 261-267 (2002-04-04)
A high-performance liquid chromatographic (HPLC) method for simultaneous determination of dehydroacetic acid (DHA), benzoic acid (BA), sorbic acid (SOA) and salicylic acid (SA) was developed for application to cosmetic products. Isocratic reversed-phase HPLC was employed for quantitative analysis using tetra-n-butylammonium
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