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Merck

808149

TSK-GEL® 反相 HPLC 色谱柱

phase C18 (octadecyl), L × I.D. 25 cm × 4.6 mm, 5 μm particle size

别名:

TSKgel® Reversed Phase HPLC Column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501

材料

stainless steel

agency

suitable for USP L1

產品線

TSKgel®

製造商/商標名

Tosoh ODS-80Tm, 08149

標籤範圍

15% Carbon, monolayer, endcapped

參數

0-100% organic solvent
0-45 °C temperature
1.2 mL/min flow rate
310 bar max. pressure

技術

HPLC: suitable

長度 × 內徑

25 cm × 4.6 mm

表面積

425 m2/g

基質

silica particle platform

基質活性組

C18 (octadecyl) phase

粒徑

5 μm

孔徑

100 Å pore diameter

工作pH值

2.0-7.5

分離技術

reversed phase

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一般說明

TSKgel ODS-80Tm is a general purpose column for the analysis of low MW pharmaceuticals, bases, nucleosides and nucleotides. Common applications include purity checks and peptide mapping. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-80Tm has 80Å nominal pore size. The pore size of the base silica is 100Å.

應用

TSKgel® ODS-80Tm HPLC Column may be used for the separation of:
  • Dehydroacetic acid, benzoic acid, sorbic acid and salicylic acid in cosmetic products during their determination by solid-phase extraction (SPE) followed by analysis using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection.
  • Cilostazol and its metabolites in human urine during their determination by liquid-liquid extraction (LLE) followed by analysis using HPLC-UV.
  • Heterocyclic amines in food products during their analysis by LC coupled to mass spectrometry with electrospray ionization (ESI-MS), LC combined with tandem mass spectrometry (MS/MS) with ESI, LC coupled to atmospheric pressure chemical ionization (APCI)-MS/MS, LC with UV-diode array detection (DAD), LC with UV-fluorescence detection (FLD) and LC in conjunction with electrochemical detection, etc.
  • Rabeprazole and its metabolite, rabeprazole thioether in human plasma samples during their analysis by column switching HPLC with UV detection.
  • β-lactam antibiotic, cefaclor during its determination by HPLC-FLD.

外觀

Shipped in 70% methanol - 30% water.

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法律資訊

TSKgel is a registered trademark of Tosoh Corporation

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分析证书(COA)

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Sensitive determination of a β-lactam antibiotic, cefaclor by liquid chromatography with chemiluminescence detection.
Kai M, et al.
Journal of Pharmaceutical and Biomedical Analysis, 30(6), 1765-1771 (2003)
Analysis of heterocyclic amines in food products: interlaboratory studies
Santos FJ, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 802(1), 69-78 (2004)
Determination of cilostazol and its metabolites in human urine by high performance liquid chromatography.
Tata PNV, et al.
Journal of Pharmaceutical and Biomedical Analysis, 24(3), 381-389 (2001)
Determination of rabeprazole and its active metabolite, rabeprazole thioether in human plasma by column-switching high-performance liquid chromatography and its application to pharmacokinetic study.
Uno T, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 824(1-2), 238-243 (2005)
E Mikami et al.
Journal of pharmaceutical and biomedical analysis, 28(2), 261-267 (2002-04-04)
A high-performance liquid chromatographic (HPLC) method for simultaneous determination of dehydroacetic acid (DHA), benzoic acid (BA), sorbic acid (SOA) and salicylic acid (SA) was developed for application to cosmetic products. Isocratic reversed-phase HPLC was employed for quantitative analysis using tetra-n-butylammonium

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