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產品線
TOYOPEARL®
形狀
powder
包裝
bottle of 10 g
製造商／商標名
Tosoh TOYOPEARL® AF, 08000
標籤範圍
800 μeq/g ligand density
參數
3 bar max. pressure
技術
HPLC: suitable
基質
hydroxylated methacrylic polymer
基質活性組
expoxy-activated phase
粒徑
65 μm
容量
>60 g/L adsorption capacity (soybean trypsin inhibitor)
分離技術
affinity
儲存溫度
2-8°C
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一般說明
Toyopearl AF-Epoxy-650 resin is an activated resin provided in dry form for the immobilization of protein ligands for affinity chromatography. It is used when high densities of low molecular weight molecules need to be attached. It is also useful when a conversion to other special functional groups is required prior to ligand immobilization. For instance, its hydrazide form is very useful for carbohydrates or glycoprotein ligands.
應用
TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.
外觀
Shipped as dry, free flowing powder.
法律資訊
Toyopearl is a registered trademark of Tosoh Corporation
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
96.8 °F - closed cup
閃點（°C）
36 °C - closed cup
S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;
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