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描述
HW-65F
產品線
TOYOPEARL®
形狀
slurry
包裝
bottle of 500 mL
參數
3 bar max. pressure
技術
HPLC: suitable
基質
hydroxylated methacrylic polymer
基質活性組
hydroxylated methacrylate phase
粒徑
30-60 μm
孔徑
10,000-1,000,000 Da MW range
工作pH值
2-13
分離技術
size exclusion (SEC)
一般說明
Toyopearl HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all Toyopearl "-650" designated products.
應用
TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.
規格
Clean in place with 0.5 M NaOH or 0.1 M HCl.
外觀
Shipped in 20% (v/v) ethanol.
法律資訊
Toyopearl is a registered trademark of Tosoh Corporation
訊號詞
Warning
危險聲明
危險分類
Flam. Liq. 3
水污染物質分類（WGK）
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
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