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Merck

66825-U

Supelco

BIOshell A400 Protein C4 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

别名:

Protein C4 Reversed-Phase Chromatography Column

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52

product name

BIOshell A400 Protein C4, 3.4 μm HPLC Column, 3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

材料

stainless steel hardware

品質等級

agency

suitable for USP L26

描述

Shell thickness (0.2 μm)
Solid Core (3.0 μm)

產品線

BIOshell

特點

endcapped

製造商/商標名

BIOshell

包裝

1 ea of

標籤範圍

0.4% carbon loading

參數

600 bar max. pressure
90 °C max. temp.

技術

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

長度 × 內徑

10 cm × 2.1 mm

表面積

15 m2/g

表面覆盖率

4.2 μmol/m2

基質

spherical silica particle platform
superficially porous particle

基質活性組

C4 (butyl) phase

粒徑

3.4 μm

孔徑

400 Å

工作pH值範圍

2-9

分離技術

reversed phase

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一般說明

BIOshell 400 Å Protein C4 columns contain 3.4 μm advanced Fused-Core particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns provide excellent separations on very hydrophobic proteins such as those found in the cell membrane. The C4 ligand combined with an exhaustively endcapped surface ensures optimum protein recovery of larger or very hydrophobic biomolecules. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C4 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 2 to 9.

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商品

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

实验方案

Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.

Optimized USP method for filgrastim, a recombinant form of human granulocyte colony stimulating factor (GCSF). Used in different biologics, it is crucial to know the purity of filgrastim.

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

HPLC Analysis of the Monoclonal Antibody (mAb) Erbitux (Cetuximab) on BIOshell™ A400 Protein C4

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相关内容

High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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