生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
2H2, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 38 kDa
observed mol wt ~55 kDa
純化經由
using Protein A
物種活性
human
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgGκ
表位序列
Extracellular domain
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
基因資訊
human ... CD86(942)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 2H2 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CD86. It targets an epitope within 16 amino acids from the extracellular domain.
免疫原
KLH-conjugated linear peptide corresponding to 16 amino acids from the extracellular domain of human CD86.
應用
Quality Control Testing
Evaluated by Western Blotting with MCF-7.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD86 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD86 in human spleen tissue lysate and a 1:50,000 dilution detected T7/HIS-tagged, human recombinant CD86.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD86 in human tonsil tissue sections.
Flow Cytometry Analysis: 1 µg from a representative lot detected CD86 in one million human peripheral blood mononuclear cells (PBMC).
Affinity Binding Assay: A representative lot of this antibody bound CD86 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD86 in HDLM-2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with MCF-7.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CD86 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD86 in human spleen tissue lysate and a 1:50,000 dilution detected T7/HIS-tagged, human recombinant CD86.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD86 in human tonsil tissue sections.
Flow Cytometry Analysis: 1 µg from a representative lot detected CD86 in one million human peripheral blood mononuclear cells (PBMC).
Affinity Binding Assay: A representative lot of this antibody bound CD86 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD86 in HDLM-2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
T-lymphocyte activation antigen CD86 (UniProt: P42081; also known as Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1) is encoded by the CD86 (also known as CD28LG2) gene (Gene ID: 942) in human. CD86 is a type I transmembrane protein belonging to the immunoglobulin superfamily, containing an extracellular Ig-like V-type (aa 33-131) and Ig-like C2-type (aa 15-225) domains essential for T cell interaction, a transmembrane domain (aa 248-268) and a cytoplasmic domain. The extracellular domain of CD86 is responsible for binding to CD28 or CTLA-4 on T cells. CD86 is expressed on the surface of antigen-presenting cells, including dendritic cells, macrophages, and B cells. Its expression is upregulated upon activation of these cells, which typically occurs during an immune response to pathogens. Its dysregulation has been implicated in autoimmune diseases such as rheumatoid arthritis and multiple sclerosis, allergic reactions, and transplant rejection, making it a potential target for immunotherapy. In some cancers, the manipulation of CD86 and its pathways is explored for therapeutic cancer vaccines.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重構
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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