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Merck
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ZRB2389

Sigma-Aldrich

Anti-CD46 Antibody, clone 1C10 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

别名:

Membrane cofactor protein;TLX;Trophoblast leukocyte common antigen

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41
共軛:
unconjugated
application:
FACS
ICC
IHC
WB
affinity binding assay
無性繁殖:
1C10, recombinant monoclonal
物種活性:
human
citations:
技術:
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

生物源

rabbit

品質等級

重組細胞

expressed in HEK 293 cells

共軛

unconjugated

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

1C10, recombinant monoclonal

描述

1C10 Clone

產品線

ZooMAb® learn more

形狀

lyophilized

分子量

calculated mol wt 43.75 kDa
observed mol wt ~55 kDa

純化經由

using Protein A

物種活性

human

包裝

antibody small pack of 25 μL

環保替代產品特色

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

加強驗證

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

技術

affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同型

IgG

表位序列

C-terminal extracellular domain

Protein ID登錄號

UniProt登錄號

環保替代類別

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... CD46(4179)

相关类别

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1C10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects CD46. It targets an epitope within 24 amino acids from the extracellular domain within the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 24 amino acids from the extracellular domain within the C-terminal half of human CD46.

應用

Quality Control Testing

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD46 in HL-60 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD46 in K562 cells.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD46 in one million Human peripheral blood mononuclear cells (PBMC).

Affinity Binding Assay: A representative lot of this antibody bound CD46 peptide with a KD of 1.3 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD46 in human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Membrane cofactor protein (UniProt: P15529; also known as TLX, Trophoblast leukocyte common antigen, CD46) is encoded by the CD46 (also known as MCP, MIC10) gene (Gene ID: 4179) in human. CD46 is a single-pass type I membrane glycoprotein that acts as a cofactor for complement factor I, a serine protease that protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It is expressed in all nucleated cells and is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 35-343), a transmembrane domain (aa 344-366), and a cytoplasmic domain (aa 367-392). The cytoplasmic domain has two tails that are termed as Cyt1 and Cyt2. CD46 also contains four Sushi domains that are required for its interaction with various human viruses. Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein; Sushi domains 2 and 3 are essential for Herpesvirus 6 binding and domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are considered to be the most important for interaction with C3b and C4b. CD46 exhibits both signaling and receptor properties in immune and nonimmune cells, most of which are dependent on the expression of its cytoplasmic tails. Activation of CD46 is reported to promote T cell activation and differentiation and co-stimulation of TCR with CD46 can lead to T cell proliferation. Activated CD46 also leads to Tr1 Treg differentiation and secretion of IL-10 and granzyme B. The Tr1 differentiation initiated by CD46 is attributed to its Cyt1 domain. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Choileain, SN., et al. (2011). PLoS One 6(1); e16287; Weyand, NJ et al. (2010). J. Immunol. 184 (2); 694-701; Weyand, NJ., et al. (2006). Infect. Immun. 74(4);2428-2435).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析证书(COA)

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