生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1J18, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 164.19 kDa
observed mol wt ~230 kDa
純化經由
using Protein A
物種活性
human
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal half
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
基因資訊
human ... CUX1(1523)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1J18 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Homeobox protein cut-like 1 (CUX1). It targets an epitope within 21 amino acids from the C-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal half of human Homeobox protein cut-like 1 (CUX1).
應用
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell nuclear extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CUX1 in HEK293 cell nuclear extract.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CUX1 in Human cerebellum tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CUX1 peptide with a KD of 6.7 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CUX1 in K562 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HEK293 cell nuclear extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CUX1 in HEK293 cell nuclear extract.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CUX1 in Human cerebellum tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CUX1 peptide with a KD of 6.7 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CUX1 in K562 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Homeobox protein cut-like 1 (UniProt: P39880; also known as CCAAT displacement protein, CDP, Homeobox protein cux-1, CCAAT displacement protein, CDP, Homeobox protein cux-1) is encoded by the CUX1 (also known as CUTL1) gene (Gene ID: 1523) in human. Homebox protein CUX1 is member of the CUT Homebox family that has a broad role in mammalian development. It is a transcription factor that is involved in the control of neuronal differentiation in the brain. It regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. It has a conserved coiled-coil region at the N terminus (aa 56-407), three internal 60-amino-acid repeats (Cut repeats; 542-629; 934-1021; and 1117-1204), and a divergent homeodomain located near the C terminus (aa 1244-1303). CUX1 serves as a repressor of developmentally regulated gene expression and acts by preventing binding of positively-acting CCAAT factors to promoters. It is also a component of NF-muNR repressor, which binds to the matrix attachment regions (MARs) (5′ and 3′) of the immunoglobulin heavy chain enhancer. CUX1 is shown to repress T-cell receptor β enhancer function by binding to the matrix attachment region beta (MARβ), which is an ATC-rich DNA sequence located upstream of the TCR β enhancer. Its cleaved product, CDP/Cux p110, is reported to play a role in cell cycle progression, in particular at the G1/S transition. While CUX1 only transiently binds to DNA and carries the CCAAT-displacement activity, CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1. Eleven different isoforms of CUX1 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity™, reproducibility, and stability over conventional monoclonals. (Ref.: Goulet, B., et al. (2004). Mol. Cell. 14(2); 207-219; Ellis, T., et al. (2001). Genes Dev. 15 (17); 2307-2319).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
重構
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
Affinity is a trademark of Mine Safety Appliances Co.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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