生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3D13, recombinant monoclonal
描述
3D13 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 98.34 kDa
observed mol wt ~140 kDa
純化經由
using Protein A
物種活性
monkey, mouse, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... AXL(558)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3D13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase receptor Axl (UFO). It targets an epitope within 23 amino acids from the C-terminal, cytoplasmic domain.
免疫原
KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, cytoplasmic domain of human Tyrosine-protein kinase receptor Axl (UFO).
應用
Quality Control Testing
Evaluated by Western Blotting in COS-7 and NIH3T3 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Axl (UFO) in COS-7 cell lysate and 1:1,000 dilution detected Axl (UFO) in NIH3T3 cell lysate.
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Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Axl in U-251 MG cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Axl (UFO) in one million U251 MG cells.
Affinity Binding Assay: A representative lot of this antibody bound Axl (UFO) peptide with a KD of 3.0 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Axl (UFO) in human skeletal muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in COS-7 and NIH3T3 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Axl (UFO) in COS-7 cell lysate and 1:1,000 dilution detected Axl (UFO) in NIH3T3 cell lysate.
.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Axl in U-251 MG cells.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Axl (UFO) in one million U251 MG cells.
Affinity Binding Assay: A representative lot of this antibody bound Axl (UFO) peptide with a KD of 3.0 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Axl (UFO) in human skeletal muscle tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Tyrosine-protein kinase receptor UFO (UniProt: P30530; also known as EC:2.7.10.1, AXL oncogene) is encoded by the AXL (also known as UFO) gene (Gene ID: 558) in human. AXL is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 26-451), a transmembrane domain (aa 452-472), and a cytoplasmic domain (aa 473-894). It serves as receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to its ligand the growth arrest specific protein 6 (GAS6) via amino acids 26-92. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL that activates its kinase activity. Its protein kinase domain is localized in amino acids 536-807. Following its activation, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3. Other known downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and PI3-kinase regulatory subunits by AXL also leads to the downstream activation of the Akt. AXL regulates several physiological processes including cell survival, cell proliferation, migration, and differentiation. The AXL/GAS6 signaling is associated with tumor growth, metastasis, invasion, epithelial-mesenchymal transition, angiogenesis, and drug resistance. Higher expression of AXL is reported in metastatic colon and breast cancer; however, its expression in normal colon tissue is weak. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhu, C., et al. (2019). Mol. Cancer. 18; Article 153; Weinger, JG., et al (2008). J. Neurochem. 106(1); 134-146; Berclaz, G., et al. (2001). Ann. Oncol. 12(6); 819-824).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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