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Key Documents

ZRB1812

Sigma-Aldrich

Anti-RARγ1 Antibody, clone 1C22 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

rabbit

品質等級

重組細胞

expressed in HEK 293 cells

共軛

unconjugated

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

1C22, recombinant monoclonal

描述

1C22 Clone

產品線

ZooMAb® learn more

形狀

lyophilized

分子量

calculated mol wt 50.34 kDa
observed mol wt ~55 kDa

純化經由

using Protein A

物種活性

human

物種活性(以同源性預測)

monkey, monkey, bovine, porcine, horse, mouse

包裝

antibody small pack of 25 μL

環保替代產品特色

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

加強驗證

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

技術

affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

同型

IgG

表位序列

N-terminal

Protein ID登錄號

UniProt登錄號

環保替代類別

運輸包裝

ambient

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... RARG(5916)

相关类别

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1C22 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Retinoic Acid Receptor 1 (RAR 1). It targets an epitope within 16 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Retinoic Acid Receptor 1 (RAR 1).

應用

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected RAR 1 in MCF-7 cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound RAR 1 peptide with a KD of 1.1 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:300 dilution from a representative lot detected RAR 1 in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Retinoic acid receptor gamma (UniProt: P13631; also known as RAR-gamma, Nuclear receptor subfamily 1 group B member 3) is encoded by the RARG (also known as NR1B3) gene (Gene ID: 5916) in human. RAR 1 is a member of the nuclear hormone receptor family that serves as a receptor for retinoic acid. Three isoforms of RAR have been described: RAR-a , RAR-β, and RAR- that are expressed in temporal- and tissue-specific patterns beginning early in development and continuing throughout life. RAR- shares about 90% homology with RAR-a and RARβ. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain (aa 90-155) and a C-terminal ligand-binding domain. It contains two NR C4-type Zinc finger domains (aa 90-110 and 126-150). Four different isoforms of RAR- have been described that are produced by alternative splicing and they differ only in their N-terminal regions. RARs form heterodimers with member of the RXR gene family that also has three different isomers of RXRs (RXR-a, RXR-β, RXR- ) and are expressed in distinct patterns. The binding of all-trans-retinoic acid to RAR in an RAR-RXR heterodimer serves to activate the transcription factor complex, which, when bound to specific retinoic acid response elements (RARE) in target genes can recruit additional protein factors that activate or repress gene expression. The activity of the RARs is further modulated by posttranslational modifications. In human epidermis, RAR- is reported to be the predominant RAR that accounts for about 87% of RAR protein and RAR-a makes up the remainder. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fisher, GJ., et al. (1998). J. Invest. Dermatol. 110(3); 297-300).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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