生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1C13, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 77.51 kDa
observed mol wt ~77 kDa
物種活性
rat, mouse, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... PRKCD(5580)
mouse ... Prkcd(18753)
rat ... Prkcd(170538)
相关类别
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1C13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects PKCd It targets an epitope within 18 amino acids from the C-terminal, AGC kinase domain.
免疫原
KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal AGC kinase domain of human protein kinase C delta ( PKCd).
應用
Anti-PKCd, clone 1C13 ZooMAb, Cat. No. ZRB1623, is a recombinant Rabbit monoclonal antibody that specifically targets PKCd and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Integrin -5 in HT-1080 and NIH3T3 cell lysates.
Flow Cytometry Analysis: 1 μg from a representative lot detected Integrin -5 in one million HelL cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Integrin -5 in human placenta tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Integrin a-5 peptide with a KD of 2.5 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,0000 dilution from a representative lot detected Integrin -5 in HeLa cell line, A431 cell line, HUVEC cell line and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Flow Cytometry Analysis: 1 μg from a representative lot detected Integrin -5 in one million HelL cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Integrin -5 in human placenta tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Integrin a-5 peptide with a KD of 2.5 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,0000 dilution from a representative lot detected Integrin -5 in HeLa cell line, A431 cell line, HUVEC cell line and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Protein kinase Cd (UniProt: Q05655; also known as EC:2.7.11.13, nPKC-delta) is encoded by the PRKCD gene (Gene ID: 5580) in human. PKC d is a ubiquitous serine/threonine kinase involved in signal transduction associated with cell proliferation, differentiation, and apoptosis. It is a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent novel PKC enzyme that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death. It is shown to negatively regulate B cell proliferation and is also involved in self-antigen induced B cell tolerance induction. Upon DNA damage, PKCd activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In the case of ER stress or DNA damage-induced apoptosis, it can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol it can trigger apoptosis by activating MAPK11 or MAPK14 and inhibiting Akt1 and decreasing the level of XIAP. Phosphorylation at three specific sites: threonine 507, serine 645, and serine 664 is required for its full activation. PKCd is proteolytically cleaved into a catalytic subunit (aa 330-676) and a regulatory subunit (aa 1-329) by caspase-3 during apoptosis which results in kinase activation. As a result of this cleavage the pseudosubstrate motif in the regulatory subunit is released from the substrate recognition site of the catalytic subunit, which enables its activation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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