推荐产品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1M8, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 36.97 kDa
observed mol wt 37 kDa
物種活性
human, rat, mouse
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... RAD51(5888)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1M8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects RAD51 protein.
免疫原
His-tagged full-length recombinant human RAD51 protein.
應用
Anti-RAD51, clone 1M8 ZooMAb, Cat. No. ZRB1492, is a recombinant Rabbit monoclonal antibody that specifically targets RAD51 and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RAD51 in HeLa and NIH3T3 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RAD51 in human testis tissue sections.
Affinity Binding Assay Analysis: 100 μg/mL from a representative lot detected RAD51 in RAD51 and GST protein showed a KD of 1.0 x 10^-12 whereas binding of ZRB1492 to non-specific GST protein showed a KD of 1.7 x 10^-3.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAD51 in HeLa, A431, HepG2, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RAD51 in human testis tissue sections.
Affinity Binding Assay Analysis: 100 μg/mL from a representative lot detected RAD51 in RAD51 and GST protein showed a KD of 1.0 x 10^-12 whereas binding of ZRB1492 to non-specific GST protein showed a KD of 1.7 x 10^-3.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RAD51 in HeLa, A431, HepG2, and NIH 3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
DNA repair protein RAD51 homolog 1 (UniProt: Q06609; also known as HsRAD51, hRAD51, RAD51 homolog A) is encoded by the RAD51 (also known as RAD51A, RECA) gene (Gene ID: 5888) in human. RAD51 plays an important role in DNA repair through homologous recombination. It is highly expressed in testis and thymus and is also found in small intestine, placenta, colon, pancreas and ovary. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. It is shown to catalyze the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. Phosphorylation of RAD51 at threonine 309 by checkpoint kinase 1 (CHEK1) enhances its association with chromatin at sites of DNA damage and promotes DNA repair. It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. RAD51 also plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress and its stress-induced increases are observed in the mitochondria. Mutations in RAD51 gene are observed in cases of invasive ductal carcinoma and in Fanconi anemia. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hilario, J., et al. (2009). Proc. Natl. Acad. Sci. USA. 106(2);361-368).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
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