推荐产品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1D4, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 87.34 kDa
observed mol wt 90 kDa
物種活性
human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... STAT1(6772)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1D4 is a a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human STAT1. It targets an epitope within 38 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 38 amino acids from the C-terminal region of human Signal transducer and activator of transcription 1-alpha/beta (STAT1) protein.
應用
Rabbit recombinant monoclonal antibody Anti-STAT1 (isoform ), clone 1D4 ZooMAb Rabbit Monoclonal, Cat. No. ZRB1459 is used in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry and Affinity Binding Assay, for the detection of STAT1.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STAT1 in HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected STAT1 in U2OS and Hela cells treated with interferon-gamma.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected STAT1 in human kidney and human breast cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant STAT1 alpha fragment with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected STAT1 in U2OS and Hela cells treated with interferon-gamma.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected STAT1 in human kidney and human breast cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant STAT1 alpha fragment with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
標靶描述
Signal transducer and activator of transcription 1-alpha/beta (UniProt: P42224; also known as Transcription factor ISGF-3 components p91/p84, STAT1) is encoded by the STAT1 gene (Gene ID: 6772) in human. STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling proteins. STAT1 is activated by many different ligands including interferons, interleukins, and growth factors. STAT1 exists in two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta, which are encoded by the same gene with splicing variant. Following ligand binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases and tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and enter the nucleus. Phosphorylation on serine 727 by several kinases including MAPK14, ERK1/2 and CAMKII following on IFN-gamma stimulation, regulates STAT1 transcriptional activity. This phosphorylation also promotes sumoylation though increasing interaction with PIAS. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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