ZRB1211

Anti-TAGLN Antibody, clone 1D10 , ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: 22 kDa actin-binding protein, Protein WS3-10, SM22-alpha, Smooth muscle protein 22-alpha, Transgelin
• 分類程式碼代碼: 12352203

属性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1D10, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 22.61 kDa; observed mol wt ~22 kDa
• 物種活性: mouse, human
• 物種活性（以同源性預測）: bovine, rat
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: Q01995
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TAGLN(6876)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1D10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Transgelin. It targets an epitope within 12 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Transgelin.

應用

Anti-TAGLN, clone 1D10 ZooMAb, Cat. No. ZRB1211, is a highly specific recombinant rabbit monoclonal antibody that detects Transgelin/SM22 alpha and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TAGLN in HEPG2, NIH-3T3, and human skeletal muscle lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TAGLN in human uterus tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TAGLN in C2C12 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

標靶描述

Transgelin (UniProt: Q01995; also known as 22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha) is encoded by the TAGLN (also known as SM22, WS3-10) gene (Gene ID:6876) in human. Transgelin is a shape change sensitive actin-binding protein of the calponin family that contains a calponin-homology domain (aa 24-137) and a C-terminal calponin-like module (aa 175-200) that is essential for actin binding. It is found in vascular and visceral smooth muscles of all adult vertebrates and is considered as an early marker of smooth muscle differentiation. Transgelin expression has also been reported in fibroblasts, spinal cord, adrenal gland, and in several immune cells. It is overexpressed in senescent human fibroblasts. It is shown to be transiently expressed in heart and skeletal muscle cells during early embryogenesis in mouse. Transgelin serves as an actin stress fiber-associated protein that stabilizes actin gels in vitro. It is reported to promote podosome formation and contributes to cell motility in response to injury or inflammation. Activation of TGF 1 signaling is shown to induce Transgelin expression, which enhances mesenchymal stem cells (MSC) differentiation and migration. Over-expression of Transgelin is shown to enhance osteoblast and adipocyte differentiation of MSC and these effects are abolished in the presence of TGF beta signaling inhibitors. Elevated levels of Transgelin are reported to promote tumor metastasis and are correlated with aggressive tumor behavior and poor prognosis in colorectal cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Assinder, SJ et al. (2009). Int. J. Biochem. Cell Biol. 41(3); 482-486; Elsafadi, M., et al. (2016). Cell Death and Disease 7; e2321; Zhou, H-M., et al. (2016). BMC Cancer. 16: 55).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1