推荐产品
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
human, chicken, rat, mouse
技術
western blot: 1:1,000 using chick embryo fibroblasts (CEFs) transfected with activated Src.
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
phosphorylation (pTyr822)
基因資訊
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
一般說明
Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.
免疫原
synthetic phosphopeptide derived from the region of vinculin that contains tyrosine 822.
應用
Anti-phospho-Vinculin (pTyr822) antibody produced in rabbit has been used in immunofluorescence staining.
生化／生理作用
Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading.In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.
外觀
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Sex-specific association of rs4746172 of VCL gene with hypertension in two Han populations from Southern China.
Yu Q
Scientific Reports (2015)
Molecular mechanism of transglutaminase-2 in corneal epithelial migration and adhesion
Tong L
Biochimica et Biophysica Acta, 1833, 1304-1315 (2013)
Shun Shibata et al.
Cell reports, 25(6), 1668-1679 (2018-11-08)
The extracellular matrix plays a key role in stem cell maintenance, expansion, and differentiation. Laminin, a basement membrane protein, is a widely used substrate for cell culture including the growth of human induced pluripotent stem cells (hiPSCs). Here, we show
Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q.
Mulligan LM
Genomics, 13, 1347-1349 (1992)
The structure and regulation of vinculin.
Ziegler WH
Trends in Cell Biology, 16, 453-460 (2006)
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