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形狀
lyophilized powder
比活性
15-30 units/mg protein (biuret)
分子量
tetramer ~32 kDa
儲存溫度
−20°C
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
InChI 密鑰
VPXDHZMLJOJKOX-UHFFFAOYSA-N
一般說明
Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.
應用
用 球状节杆菌 产生的尿酸酶涂覆电极制成生物传感器,可检测尿样中的尿酸。它也可用于分析 Zn、Cu、Mg 和 Mn 离子对尿酸酶的影响。
生化/生理作用
球形节杆菌是家禽粪便和垃圾中尿酸酶或尿酸氧化酶的主要产生菌。尿酸酶可水解尿酸,引起禽粪氨挥发。在大多数哺乳动物中,尿酸酶将尿酸转化为尿囊素(一种可溶性排泄产物)。人类缺乏尿酸酶是由于在原始人类进化过程中丢失了该酶。由于这增加了尿酸循环水平,人类罹患痛风、肾结石、高血压和糖尿病的风险也随之加大。
Zn 和 Cu 显示能抑制尿酸酶活性。
分子量: 4×~32 kDa (四聚体)
等电点:4.64
K M 值(以尿酸为底物):~0.01 mM
活性的最适 pH:8.5-9.5
稳定性最佳 pH 值:6.0-9.5
等电点:4.64
K M 值(以尿酸为底物):~0.01 mM
活性的最适 pH:8.5-9.5
稳定性最佳 pH 值:6.0-9.5
單位定義
在25℃、pH 9.0条件下,一个单位每分钟可将 1.0μ摩尔尿酸转化为尿囊素。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Uric acid: a danger signal from the RNA world that may have a role in the epidemic of obesity, metabolic syndrome, and cardiorenal disease: evolutionary considerations.
Seminars in Nephrology, 31(5), 394-399 (2011)
Poultry science, 82(2), 223-231 (2003-03-07)
Inhibition of microbial uricase in poultry manure is critical to reduce NH3 volatilization, because hydrolysis of uric acid by microbial uricase is the first step in the production of NH3 gas in poultry manure. Three experiments were conducted to evaluate
Development of a sandwich format, amperometric screen-printed uric acid biosensor for urine analysis.
Analytical biochemistry, 428(1), 39-43 (2012)
Uric acid and evolution.
Rheumatology (Basel), 49(11), 2010-2015 (2010)
Effect of minerals on activity of microbial uricase to reduce ammonia volatilization in poultry manure.
Poultry Science, 82(2), 223-231 (2003)
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