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Merck

TRUE9

Sigma-Aldrich

TrueGel3D 水凝胶试剂盒

SLO-PVA, PEG non cell-degradable crosslinker

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

運輸包裝

dry ice

儲存溫度

−70°C

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一般說明

The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.

應用

TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.

特點和優勢

  • Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
  • Flexibility in modulating gel stiffness
  • Does not allow cell migration
  • Does not allow cell recovery and post-culture analyses
  • Cell inert hydrogel (if not customized with RGD peptide)

成分

TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.

注意

Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.

象形圖

Corrosion

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3


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Design of Spherically Structured 3D In vitro Tumor Models-Advances and Prospects
Ferreira LP, et al.
Acta Biomaterialia (2018)

商品

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

TrueGel3D™ is a synthetic hydrogels platform for 3D cell culture that aim to mimic the natural extracellular environment for more physiological culture conditions

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

实验方案

TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits

相关内容

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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