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T7024

Sigma-Aldrich

四甲基乙二胺

BioReagent, for molecular biology, ≥99% (GC)

别名:

1,2-双(二甲基氨基)乙烷, TEMED, TMEDA

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About This Item

线性分子式:
(CH3)2NCH2CH2N(CH3)2
CAS号:
110-18-9
分子量:
116.20
Beilstein:
1732991
EC號碼:
203-744-6
MDL號碼:
MFCD00008335
分類程式碼代碼:
41105319
PubChem物質ID:
24900242
NACRES:
NA.31

等級

for molecular biology

品質等級

200

產品線

BioReagent

化驗

≥99% (GC)

expl. lim.

9.08 %

折射率

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp

−55 °C (lit.)

密度

0.775 g/mL at 20 °C (lit.)

異物活動

DNase, RNase, protease, none detected

SMILES 字串

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChI 密鑰

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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應用

本产品被指定为分子生物学级，适用于聚丙烯酰胺凝胶电泳（PAGE）。经过分析，不含核酸酶和蛋白酶。
TEMED 是一种叔胺，用于催化过硫酸铵或核黄素形成自由基。TEMED 产生的自由基导致化合物丙烯酰胺和双丙烯酰胺聚合，并形成凝胶基质聚丙烯酰胺。因为 TEMED 仅以其游离碱形式起作用，所以低 pH 条件将抑制聚合。,,
已经研究了 TEMED对银染 SDS PAGE 中背景升高的潜在作用。 TEMED 已经用于在毛细管中涂覆软聚合物（例如聚丙烯酰胺），用于通过原子力显微镜研究。 使用 TEMED 制备含有固定在凝胶垫内的寡核苷酸和蛋白质的微芯片。
通过使用 TEMED 研究了蛋白质的毛细管等电聚焦（等电点（pI）> 12）。 TEMED 已用于小有机分子的 HPLC。

數量

针对 50mL 丙烯酰胺凝胶溶液，建议用量为 35μL。

訊號詞

Danger

危險分類

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

61.7 °F - closed cup

閃點（°C）

16.5 °C - closed cup

個人防護裝備

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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H Rosing et al.
Journal of chromatography. B, Biomedical applications, 668(1), 107-115 (1995-06-09)
A sensitive high-performance liquid chromatographic (HPLC) assay has been developed and validated for the quantitation of the novel anticancer agent topotecan and topotecan as the total of its lactone and carboxylate forms in human plasma. Linear response in analyte standard
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
Deepa Mohan et al.
Journal of chromatography. A, 979(1-2), 271-276 (2002-12-25)
The non-availability of commercial carrier ampholytes in the pH range greater than 11 has contributed to difficulties in focusing and resolving highly basic proteins/peptides using capillary isoelectric focusing (cIEF). Two different approaches, involving the use of N,N,N',N'-tetramethylethylenediamine (TEMED) and ampholyte
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
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