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生物源
human
重組細胞
expressed in E. coli
籤條
GST tagged
化驗
≥95% (SDS-PAGE)
形狀
lyophilized
分子量
~52 kDa
包裝
pkg of 100 μg
儲存條件
avoid repeated freeze/thaw cycles
技術
activity assay: suitable
顏色
white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... ANXA5(308)
一般說明
Annexin V is a member of the annexin family of calcium and membrane-binding proteins that have anticoagulatory and anti-inflammatory properties. The encoded protein is 320 amino acid long and is folded into four domains, each having five α-helices, forming a superhelical globular structure. The ANX5 gene is mapped to human chromosome 4q26-q28 and spans a length of 28kb containing 13 exons and 12 introns.
應用
Annexin V has been used to examine phosphorylation activity of ChaK1 (channel-kinase 1).
生化／生理作用
Annexins are a family of water-soluble proteins that bind to membranes and phospholipids in a calcium-dependent manner. They participate in several cellular functions, such as membrane fusion and cytoskeletal interaction. Annexin V is a potent anticoagulant and serves as a voltage-gated calcium channel when bound to membrane in vitro. It is found to inhibit phospholipase A2 and protein kinase C.
外觀
Lyophilized from PBS, pH 7.4.
重構
Reconstitute with 100 μL deionized water.
其他說明
Recombinant human annexin V fused to a GST-tag.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Marielle E Yohe et al.
Science translational medicine, 10(448) (2018-07-06)
The RAS isoforms are frequently mutated in many types of human cancers, including PAX3/PAX7 fusion-negative rhabdomyosarcoma. Pediatric RMS arises from skeletal muscle progenitor cells that have failed to differentiate normally. The role of mutant RAS in this differentiation blockade is
Xuerui Zhang et al.
Oncotarget, 8(26), 42602-42612 (2017-04-14)
Annexin V, a protein with high affinity to phosphatidylserine (PS) in a calcium dependent manner, has been widely used to probe apoptosis. Annexin V in inhibiting engulfment of apoptotic cells by macrophages had been reported to increase the immunogenicity of
Jesse D Gelles et al.
Cell death & disease, 7(12), e2493-e2493 (2016-12-03)
Quantitative and kinetic analyses of apoptotic cell death are integral components of exploring cell biology, measuring cellular stress responses, and performing high-throughput genomic/RNAi/drug screens. Here, we present a detailed method that integrates robust kinetic real-time high-content imaging with Annexin V
Zhiqiang Wei et al.
Chinese medicine, 15, 12-12 (2020-02-07)
Osteoarthritis (OA) is a metabolic disorder and able to be relieved by traditional Chinese medicines. However, the effect of Ligusticum wallichii on OA is unknown. Cytokine IL-1β and L. wallichii extracts were used to stimulate the primary mouse chondrocytes. MTT
Phosphorylation of annexin I by TRPM7 channel-kinase.
Dorovkov MV
The Journal of Biological Chemistry, 279, 50643-50646 (2004)
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