SRP6546

Procathepsin L human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 别名: CTSL, CTSL1
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-PAGE)
• 形狀: liquid
• 分子量: 39.4 kDa (18-333 aa + N-terminal poly-his tag.)
• 包裝: pkg of 1 mg; pkg of 100 μg
• 濃度: 1 mg/mL
• UniProt登錄號: P07711
• 運輸包裝: wet ice
• 儲存溫度: −70°C
• 基因資訊: human ... CTSL1(1514)

说明

一般說明

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

外觀

1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.

安全信息

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable