SRP0127

DNMT2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• 别名: DNA (cytosine-5-)-methyltransferase 2, PUMET, RNMT1, tRNA (cytosine-5-)-methyltransferas, tRNA aspartic acid methyltransferase 1
• 分類程式碼代碼: 12352200
• NACRES: NA.32

属性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥80% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 71 kDa
• 包裝: pkg of 10 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_004412
• UniProt登錄號: Q6ICS7
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... TRDMT1(1787)

说明

一般說明

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) is a highly conserved protein which is part of the DNA methyltransferase family. It possesses a conserved cysteine residue in its catalytic pocket. The gene encoding it is localized on human chromosome 10p14->p12.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化／生理作用

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) methylates the cytosine-38 residue of the aspartic acid transfer RNA (tRNA-Asp). It has been shown to interact with the anticodon stem and loop. The protein also functions in cellular physiology and stress response. It is significantly expressed in cancers.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.