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一般說明
This recombinant human calprotectin is a heterodimer composed of tagged versions of both S100A8 and S100A9, where a 6xHis-SUMO tag is added to the N-terminus of S100A9, and a 6xHIS tag is added to the N-terminus of S100A8. This tagged version was found to have enhanced stability as compared to untagged versions of this protein. Removal of the tags can be achieved using HRV-3C protease (SAE0045 or SAE0110).
應用
Fecal Calprotectin is often used as a biomarker to identify inflammation in gastrointestinal disease1. This product is a recombinant version of the human protein found endogenously.
生化／生理作用
Calprotectin is a calcium and zinc binding protein that belongs to the S100 family. It is a heterodimer protein composed of S100A8 and S100A9.1 The protein is expressed in myeloid cells and in circulating monocytes and neutrophils, where it accounts for 60% of the cytosolic protein content. Calprotectin was found to play major roles in inflammation and the immune response by activation of NF-kB pathway via TLR4 and AGER,2 and its antimicrobial activity possibly through chelating of Zn.3 High concentrations of calprotectin are found and serve as a biomarker in rheumatoid, cystic fibrosis, inflammatory bowel disease and in Crohn′s disease.4 A meta-analysis also identified calprotectin as a potential biomarker for COVID-19.5
外觀
The product is lyophilized from a sterile 0.2 µm filtered solution containing 12.5 mM Tris-Cl (pH 8.0), 150 mM NaCl, and 1 mM DTT.
準備報告
Reconstitute lyophilized vial in DMEM or in PBS to a concentration up to 500 µg/mL. Spin down to collect material. Reconstituted product should be stored in working aliquots at –20 °C. Do not store in a frost-free freezer. Avoid freeze-thaw cycles.
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
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