推荐产品
生物源
alpaca
重組細胞
expressed in HEK 293 cells (Human cell line)
共軛
horse radish peroxidase (HRP)
抗體表格
affinity purified immunoglobulin
抗體產品種類
secondary antibodies
無性繁殖
recombinant monoclonal
形狀
lyophilized solid
分子量
100 kDa
物種活性
Alpaca, mouse
技術
ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000
尺寸
0.1 mL
顏色
colorless
同型
VHH
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
Goat Anti-Mouse IgG1(Fcγ) HRP Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgG1 Fcγ fragment. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG1 Fcγ fragment coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Mouse IgG1(Fcγ) HRP Alpaca Heavy chain only Antibody reacts with the Fc fragment of mouse IgG1 selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特異性
Mouse IgG1(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
免疫原
Recombinant Alpaca single variable domain secondary nano antibody
包裝
100 μg in Glass bottle
外觀
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
重構
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
儲存和穩定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C
其他說明
It has weak cross reaction with mouse IgG3. No cross-reactivity with mouse IgG2b/2c, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
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訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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