推荐产品
生物源
mouse
品質等級
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
92A, monoclonal
形狀
buffered aqueous solution
分子量
~15 kDa
物種活性
human
濃度
~1 mg/mL
技術
immunoblotting: 0.1-0.2 μg/mL using β2-Microglobulin from human urine.
immunoprecipitation (IP): 2.5-5 μg/test using lysate of human HeLa cell line.
indirect ELISA: 0.2-0.4 μg/mL using 1 μg/ml β2-Microglobulin from human urine for coating.
同型
IgG2a
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... B2M(567)
一般說明
B2M (β2-Microglobulin) is the light chain of the major histocompatibility class (MHC) I molecule expressed on the cell surface of all nucleated cells, including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells and is absent in erythrocytes. B2M is filtered during glomerular filtrations and re-absorbed and catabolized by proximal renal tubule. While under normal conditions the B2M is undetectable in urine, its increased urinary excretion has been observed to be an early marker of tubular injury in a number of settings, including nephrotoxicant exposure, cardiac surgery and renal transplantation, preceding rises in serum creatinine by as many as 4-5 days. B2M may also serve as an early biomarker for AKI (Acute Kidney Injury). B2M plays a vital role in cell survival, proliferation and metastasis in various types of cancer. Its levels are also linked with multiple myeloma (MM) and non-Hodgkin′s lymphoma (NHL). NHL patients showing higher levels of B2M have a poor disease prognosis and a higher mortality risk. The protein has a predominantly β-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In patient subjected to long-term hemodialysis, B2M is responsible for systemic amyloidosis. The levels of this protein are also altered in the cerebrospinal fluid of patients with neurological diseases, such as leptomeningeal metastasis, purulent meningitis, viral meningitis or encephalitis and neuroborreliosis. Over-expression of serum B2M is observed in hepatitis C virus (HCV) related chronic liver diseases and thus acts as a potential marker for the HCV disease progression towards cirrhosis and carcinoma.
免疫原
Recombinant B2M protein.
應用
Immunoblotting: a working concentration of 0.1-0.2 μg/mL is recommended using β2-Microglobulin from human urine.
Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.
Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.
Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.
Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.
其他說明
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
M C Chapelsky et al.
Drug safety, 7(4), 304-309 (1992-07-01)
This study assessed the potential nephrotoxicity of clarithromycin in comparison with gentamicin and placebo. Increased urinary excretion of alanine aminopeptidase (AAP) and N-acetyl-beta-D-glucosaminidase (NAG) served as markers of renal tubular injury. The study utilised a multiple-dose, double-blind, randomised, parallel group
N E Tolkoff-Rubin et al.
Clinics in laboratory medicine, 8(3), 507-526 (1988-09-01)
Traditional methods of noninvasively evaluating patients for renal injury do not accomplish the following tasks: reliably distinguish potentially treatable forms of acute renal failure from acute tubular necrosis; provide a sensitive indicator of early allograft rejection in renal transplant recipients
Philip R Greipp et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 23(15), 3412-3420 (2005-04-06)
There is a need for a simple, reliable staging system for multiple myeloma that can be applied internationally for patient classification and stratification. Clinical and laboratory data were gathered on 10,750 previously untreated symptomatic myeloma patients from 17 institutions, including
Stefan Schaub et al.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 5(4 Pt 1), 729-738 (2005-03-12)
Our aim is to develop noninvasive tests to monitor the renal allograft posttransplant. Previously, we have reported that an unbiased proteomic-based approach can detect urine protein peaks associated with acute tubulointerstitial renal allograft rejection. Identification of these proteins peaks by
M G Dehne et al.
Der Anaesthesist, 44(8), 545-551 (1995-08-01)
After cardiac surgery, transient renal dysfunction often occurs. Regional differentiation of these processes is possible only using invasive techniques, including renal biopsy. Approximately 30 different plasma protein components have been identified in the urine of healthy individuals by means of
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