生物源
mouse
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
FN-3E2, monoclonal
形狀
buffered aqueous solution
分子量
~220-260 kDa
物種活性
chicken, rat, human, mouse
技術
immunoblotting: suitable
immunofluorescence: 1:2,000-1:4,000 using human foreskin fibroblast Hs68 cells
immunohistochemistry: suitable
同型
IgM
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... FN1(2335)
一般說明
Anti-Cellular Fibronectin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the FN-3E2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the antigens released in culture from a breast cancer cell line. Fibronectin (FN), also known as cold-insoluble globulin (CIG), is an extracellular matrix multi-domain glycoprotein composed of two nearly identical disulphide bound polypeptides. Fibronectin 1 is a glycoprotein, that is coded by FN1 gene. It is expressed in the plasma and at the cell surface. It is mapped to human chromosome 2q35. Cellular fibronectin is produced by various cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells, and myocytes.
免疫原
Antigens released in culture from a breast cancer cell line
應用
Anti-Cellular Fibronectin antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
Anti-cellular Fibronectin antibody, mouse monocloneal has been used in various immunochemical techniques including immunoblotting (220-260 kDa), immunofluorescence, and immunohistochemistry.
生化／生理作用
Fibronectin participates in cell adhesion, growth, migration, wound healing, blood coagulation and metastasis. Mutations in FN1 results in glomerulopathy. It plays an important role in cell attachment and spreading, control of cell cytoskeleton, morphology and differentiation. FN1 is also involved in extracellular matrix formation, haemostasis and thrombosis. It is a ubiquitous and essential component of the extracellular matrix (ECM) and plays a vital role in tissue repair mechanism.
外觀
The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains fetal calf serum.
其他說明
This product is for R&D use only, not for drug, household, or other uses.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
The extracellular matrix proteins laminin and fibronectin contain binding domains for human plasminogen and tissue plasminogen activator.
Moser TL, et al.
The Journal of Biological Chemistry, 268(25), 18917-18923 (1993)
Receptors for cold-insoluble globulin (plasma fibronectin) on human monocytes.
Bevilacqua MP, et al.
The Journal of Experimental Medicine, 153(1), 42-60 (1981)
E L George et al.
Development (Cambridge, England), 119(4), 1079-1091 (1993-12-01)
To examine the role of fibronectin in vivo, we have generated mice in which the fibronectin gene is inactivated. Heterozygotes have one half normal levels of plasma fibronectin, yet appear normal. When homozygous, the mutant allele causes early embryonic lethality
R Manabe et al.
The Journal of cell biology, 139(1), 295-307 (1997-10-06)
Fibronectin (FN) has a complex pattern of alternative splicing at the mRNA level. One of the alternatively spliced segments, EDA, is prominently expressed during biological processes involving substantial cell migration and proliferation, such as embryonic development, malignant transformation, and wound
Yong Mao et al.
Matrix biology : journal of the International Society for Matrix Biology, 24(6), 389-399 (2005-08-03)
The extracellular matrix provides a framework for cell adhesion, supports cell movement, and serves to compartmentalize tissues into functional units. Fibronectin is a core component of many extracellular matrices where it regulates a variety of cell activities through direct interactions
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