推荐产品
生物源
mouse
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
SC-35, monoclonal
形狀
buffered aqueous solution
分子量
~35 kDa
物種活性
human, mouse, quail, hamster, frog, porcine, rat
濃度
~1.0 mg/mL
技術
ELISA: suitable
dot blot: suitable
immunoblotting: suitable
immunofluorescence: 0.125-0.25 μg/mL using HeLa cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... SRSF2(6427)
一般說明
小鼠抗-剪接因子SC-35单克隆抗体（小鼠IgG1同种型）来源于小鼠骨髓瘤细胞与部分纯化剪接体免疫的RBF-DHJ小鼠脾细胞融合产生的SC-35杂交瘤。剪接因子SC-35，也称为丝氨酸精氨酸二肽富含性剪切因子2(SRSF2)，属于丝氨酸精氨酸二肽富含性(SR)蛋白家族。
免疫原
HeLa细胞制备的部分纯化剪接体
應用
抗-剪接因子SC-35抗体已用于
- 斑点印迹
- 酶联免疫吸附测定(ELISA)
- 免疫荧光
- 免疫组织化学
- 免疫印迹
- 免疫沉淀
生化／生理作用
剪接因子SC35是pre-mRNA组成性和选择性剪接的重要调控因子。它控制基因毒性应激下的细胞凋亡。丝氨酸/精氨酸蛋白激酶1(SRPK1)可特异性磷酸化SC-35，导致p53抑制和周期蛋白D1上调。SC-35浓度升高与肺鳞状细胞癌和肺腺癌等多种癌症的侵袭性表型有关。SC35在人乳头瘤病毒(HPV)感染细胞的肿瘤进展中起致癌作用。
外觀
溶于0.01 M磷酸盐缓冲液（pH 7.4）中，含有15 mM叠氮化钠。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Valerie Edmond et al.
The EMBO journal, 30(3), 510-523 (2010-12-16)
SRSF2 is a serine/arginine-rich protein belonging to the family of SR proteins that are crucial regulators of constitutive and alternative pre-mRNA splicing. Although it is well known that phosphorylation inside RS domain controls activity of SR proteins, other post-translational modifications
E2F1 controls alternative splicing pattern of genes involved in apoptosis through upregulation of the splicing factor SC35
Merdzhanova L, et al.
Cell Death and Differentiation, 15(12), 1815-1815 (2008)
D L Spector et al.
The EMBO journal, 10(11), 3467-3481 (1991-11-01)
SC-35 is a non-snRNP spliceosome component that is specifically recognized by the anti-spliceosome monoclonal antibody alpha SC-35. In this paper we provide direct evidence that SC-35 is an essential splicing factor and we examine the immunolocalization of SC-35 by confocal
M Carmo-Fonseca et al.
The EMBO journal, 10(7), 1863-1873 (1991-07-01)
The in vivo distribution of snRNPs has been analysed by microinjecting fluorochrome-labelled antisense probes into the nuclei of live HeLa and 3T3 cells. Probes for U2 and U5 snRNAs specifically label the same discrete nuclear foci while a probe for
X D Fu et al.
Nature, 343(6257), 437-441 (1990-02-01)
A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with
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