生物源
rabbit
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
mouse
技術
immunoprecipitation (IP): 2-4 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
western blot: 1:1,000-1:2,000 using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
phosphorylation (pTyr72)
基因資訊
mouse ... Cd247(12503)
一般說明
T cell antigen receptor ζ chain (CD3ζ), also known as CD247 gene, spanning 88kb of genomic DNA, is mapped to human chromosome 1q24.2.
The T cell antigen receptor-CD3 (TCR-CD3) multimeric complex is composed of TCRαβ, CD3γε, CD3δε and ζ ζ dimers. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs.
免疫原
synthetic phosphopeptide corresponding to residues surrounding Tyr72 of mouse CD3ζ, conjugated to KLH. The corresponding sequence differs by a single amino acid in human.
應用
Anti-phospho-CD3ζ (pTyr72) antibody has been used in
- immunoblotting
- immunoprecipitation
- western blotting
生化／生理作用
T cell antigen receptor ζchain (CD3ζ) functions as a key signal transduction component of the T cell antigen receptor (TCR) complex. The encoded protein facilitates optimal effector T-cell function by enhancing receptor expression and signaling. Mutation in the gene increases the risk of susceptibility to systemic lupus erythematosus (SLE). Reduced expression of the gene has been observed in T-cells of cancer, lupus and chronic infectious diseases such as leprosy and tuberculosis patients. CD3ζ serves as a potent biomarker for determining progression and severity in patients with type 2 diabetes.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Crippling of CD3-zeta ITAMs does not impair T cell receptor signaling
Ardouin L, et al.
Immunity, 10(4), 409-420 (1999)
An immunoproteomic approach to characterize the CAR interactome and signalosome
Ramello M C, et al.
Science Signaling, 12(568), eaap9777-eaap9777 (2019)
Genetic Association of CD247 (CD3?) with SLE in a Large-Scale Multiethnic Study
Martins M
Genes and Immunity, 16, 142-150 (2015)
CD247, a Novel T Cell?Derived Diagnostic and Prognostic Biomarker for Detecting Disease Progression and Severity in Patients With Type 2 Diabetes
Eldor R
Diabetes Care, 38, 113-118 (2015)
Regulation of T Cell Receptor CD3? Chain Expression byL-Arginine
Rodriguez PC
The Journal of Biological Chemistry, 277, 21123-21129 (2002)
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