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生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
~81 kDa
物種活性
rat, mouse, human
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
~1.5 mg/mL
技術
western blot: 1.5-3.0 μg/mL using extracts of postnatal mouse brain (S1 fraction), postnatal rat brain (S1 fraction) and of HEK-293T cells over-expressing human DCLK1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... DCLK1(9201)
mouse ... Dclk1(13175)
rat ... Dclk1(83825)
一般說明
Anti-DCLK1 is produced in rabbit using as immunogen a synthetic peptide corresponding to human DCLK1, conjugated to KLH. DCLK1 (doublecortin like kinase 1) is also known as DCLK, DCAMKL1, DCDC3A and CLICK1. DCLK1 consists of an N-terminal doublecortin (DC) domain, responsible for its localization to microtubules and a C-terminal Ser/Thr kinase domain.
DCLK1 is a neuronal transmembrane enzyme that may regulate cortical development, nerve cell migration and microtubule polymerization. This enzyme is similar to DCX, but DCLK1 additionally functions as a Ca2+/calmodulin-dependent protein kinase that can phosphorylate myelin basic protein and itself . Anti-DCLK1 antibody is specific for human, rat and mouse DCLK1. In immunoblotting, detection of the DCLK1 band is specifically inhibited by the DCLK1 immunizing peptide.
免疫原
The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse DCLK1.
應用
Anti-DCLK1 antibody is suitable for use in western blot (1.5-3.0 μg/mL using extracts of postnatal mouse brain (S1 fraction), postnatal rat brain (S1 fraction) and HEK-293T cells over-expressing human DCLK1). The antibody may also be used for immunoblot (approx. 81 kDa) assays.
生化／生理作用
Doublecortin-like kinase1 (DCLK1) is widely expressed in post-mitotic neurons in the developing nervous system and important for neuronal migration to cerebral cortex. DCLK1 is highly expressed during embryogenesis and is localized to neuronal growth cones where it associates with the microtubule cytoskeleton and regulates microtubule polymerization. DCLK1 kinase domain is susceptible to cleavage by the Ca2+ -dependent protease calpain, suggesting that a Ca2+ -responsive mechanism is involved in regulating the DCLK1 kinase activity during embryogenesis. DCLK1 has been identified as a putative intestinal stem cell marker.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Cleavage of Doublecortin-like Kinase by Calpain Releases an Active Kinase Fragment from a Microtubule Anchorage Domain
Burgess H A and Reiner O
The Journal of biological chemistry, 276(39), 36397-36403 (2001)
DCAMKL1 encodes a protein kinase with homology to doublecortin that regulates microtubule polymerization
Lin P T, et al.
The Journal of Neuroscience, 20(24), 9152-9161 (2000)
Expression, characterization, and gene knockdown of zebrafish doublecortin-like protein kinase
Shimomura S, et al.
Archives of Biochemistry and Biophysics, 463(2), 218-230 (2007)
Alternative transcripts of Dclk1 and Dclk2 and their expression in doublecortin knockout mice
Tuy F P D, wt al.
Developmental Neuroscience, 30(1-3), 171-186 (2008)
N Matsumoto et al.
Genomics, 56(2), 179-183 (1999-03-03)
Human DCAMKL1, also known as KIAA0369, is a homologue of DCX (Xq22. 3), a gene associated with X-linked lissencephaly and subcortical band heterotopia. This suggests that DCAMKL1 may play a role in neuronal migration. The gene also shows similarity to
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