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SAB4200146

Sigma-Aldrich

Anti-AMSH-LP / STAMBPL1 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody

别名:

Anti-ALMalpha, Anti-AMSH-FP, Anti-AMSH-like protease, Anti-STAM binding protein-like 1, Anti-associated molecule with the SH3 domain of STAM (AMSH) like protein

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen ~50 kDa

物種活性

monkey, canine, human, rat, bovine, mouse

包裝

antibody small pack of 25 μL

濃度

~1 mg/mL

技術

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
western blot: 2.5-5.0 μg/mL using whole extracts of mouse NIH-3T3 cells
western blot: 5-10 μg/mL using using whole extracts of rat NRK cells

UniProt登錄號

Q96FJ0

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... STAMBPL1(57559)
mouse ... Stambpl1(76630)

相关类别

一般說明

AMSH-like protein (AMSH-LP), also known as STAM-binding protein-like 1 (STAMBPL1), is a close homolog of AMSH (Associated Molecule with the SH3 domain of STAM). AMSH and AMSH-LP belong to the JAMM (JAB1/MPN/Mov34 metalloenzyme) domain metalloprotease family of Zn2+-dependent deubiquitinating enzymes (DUBs). AMSH-LP contains a nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain, and a Jab1/MPN domain metalloenzyme (JAMM) motif.

應用

Anti-AMSH-LP / STAMBPL1 (C-terminal) antibody has been used in
  • immunoblotting
  • immunofluorescence
  • western blotting

生化／生理作用

AMSH-like protein (AMSH-LP) and Associated Molecule with the SH3 domain of STAM (AMSH) proteins are involved in the deubiquitination of endosomal proteins and specifically cleave K-63-linked (Lys-63) polyubiquitin chains. AMSH-LP, interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. AMSH-LP, interacts with clathrin heavy chain and this interaction is essential for its endosomal localization. It also positively regulatestransforming growth factor beta (TGF-β) signaling through interaction with inhibitory I-SMADs (inhibitory-Mothers against decapentaplegic).

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Identification of AMSH-LP containing a Jab1/MPN domain metalloenzyme motif
Kikuchi K, et al.
Biochemical and Biophysical Research Communications, 306(3), 637-643 (2003)
In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation
Gillingham A, et al.
eLife, 8 (2019)
AMSH is an endosome-associated ubiquitin isopeptidase
McCullough J, et al.
The Journal of cell biology, 166(4), 487-492 (2004)
Clathrin anchors deubiquitinating enzymes, AMSH and AMSH-like protein, on early endosomes
Nakamura M, et al.
Genes Cells, 11(6), 593-606 (2006)
Alison K Gillingham et al.
eLife, 8 (2019-07-12)
The GTPases of the Ras superfamily regulate cell growth, membrane traffic and the cytoskeleton, and a wide range of diseases are caused by mutations in particular members. They function as switchable landmarks with the active GTP-bound form recruiting to the

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