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Merck

SAB4200084

Sigma-Aldrich

Monoclonal Anti-AGO1 antibody produced in rat

~1 mg/mL, clone 4B8, purified immunoglobulin

别名:

Anti-Argonaute-1, Anti-Eukaryotic Translation Initiation Factor 2c, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rat

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

4B8, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~95 kDa

物種活性

human, canine, mouse, monkey, bovine

濃度

~1 mg/mL

技術

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using HEK-293T cell extracts

同型

IgG2a

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... EIF2C1(26523)
mouse ... Eif2c1(236511)

一般說明

Monoclonal Anti-AGO1 (rat IgG2a isotype) is derived from the hybridoma 4B8 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant human AGO1 fusion protein. The Argonaute family of proteins can be subdivided into the Ago subfamily and the Piwi subfamily. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. In human, the Ago subfamily consists of hsAgo1−4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1 is also known to be associated with Golgi and with endoplasmic reticulum. The gene is located on chromosome 1. Eukaryotic initiation factor (EIF2C1)/hAgo1 is expressed in low to medium levels in most tissues, but its expression is particularly high in embryonic kidney and lung.

免疫原

recombinant human AGO1 fusion protein.

應用

  • Monoclonal Anti-AGO1 antibody produced in rat has been used in:
  • western blotting
  • immunoprecipitation
  • chromatin immunoprecipitation(CHIP) assay
  • RNA immunoprecipitation
  • RNA-nChIP experiments

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

生化/生理作用

The Argonaute proteins are evolutionarily conserved between species and have been implicated in both transcriptional and post-transcriptional gene silencing. Eukaryotic initiation factor (EIF2C1)/argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene WT1.The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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访问文档库

Argonaute proteins couple chromatin silencing to alternative splicing
Ameyar-ZM, et al.
Nature Structural and Molecular Biology, 19(10), 998-998 (2012)
Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis
Chen Z, et al.
The Journal of Clinical Investigation, 123(3), 1057-1057 (2013)
Argonaute protein as a linker to command center of physiological processes
Wei K, et al.
Chinese Journal of Cancer Research = Chung-kuo Yen Cheng Yen Chiu, 25(4), 430-430 (2013)
RNA activating-double stranded RNA targeting flt-1 promoter inhibits endothelial cell proliferation through soluble FLT-1 upregulation
Choi S, et al.
Testing, 13(3), e0193590-e0193590 (2018)
The WEE1 regulators CPEB1 and miR-15b switch from inhibitor to activators at G2/M
Kratassiouk G, et al.
Cell Cycle, 15(5), 667-677 (2016)

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