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Merck

SAB4200041

Sigma-Aldrich

Anti-Talin antibody,Mouse monoclonal

clone TA205, purified from hybridoma cell culture

别名:

Anti-TLN

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

unconjugated

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

TA205, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~225 kDa

物種活性

chicken, bovine, human

濃度

~1.0 mg/mL

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect immunofluorescence: 0.5-1 μg/mL using HeLa cells
western blot: 0.5-1.0 μg/mL using HeLa/Jurkat cell extracts

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

chicken ... Tln1(395194)
human ... TLN1(7094)

一般說明

Monoclonal Anti-Talin (mouse IgG1 isotype) is derived from the hybridoma TA205 produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with partially purified preparation of human platelet talin. Talin is present in areas of cell-substratum and cell-cell contacts. Two types of talin were found in human, talin 1 and talin 2. Talin 1 co-distributes with integrins in the cell surface membrane. The C-terminus comprises binding sites for proteins such as β-1-integrin, actin, and vinculin. The N-terminus of the protein consists of elements for localization to cell extracellular matrix junctions. Talin 2 has a different pattern of expression compared to talin 1, but talin1 associates with unique transmembrane receptors to form novel linkages between extracellular matrices and actin cytoskeleton.
Talin 1 (TLN1) gene mapped to human chromosome 9p13.3, codes for a 270kDa cytoskeletal protein containing 2541 amino acids. Talin-1 is widely expressed in the kidney, liver, spleen, stomach, lung and vascular smooth muscle. The TLN1 antiparallel dimeric structure is characterized with a globular head containing four-point-one, ezrin, radixin, moesin (FERM) domain and rod domain involved in dimer formation.

免疫原

partially purified preparation of human platelet talin

應用

Monoclonal Anti-Talin antibody produced in mouse has been used in western blotting and immunostaining.
Monoclonal Anti-Talin antibody produced in mouse has been used in:
  • Immunoprecipitation
  • immunofluorescence
  • immunocytochemistry

生化/生理作用

Talin 1 (TLN1) is a cytoskeletal protein, which plays a vital role in cancer cell adhesion, migration and invasion. It is also involved in integrin activation and it facilitates the adhesion of integrin to actin cytoskeleton. TLN1 might act as a potential prognostic biomarker in nasopharyngeal carcinoma (NPC). Overexpression of TLN1 is observed in endometrioid carcinoma, oral squamous cell carcinoma (OSCC), prostate cancer and hepatocellular carcinoma. Serum TLN1 functions as a potential biomarker of hepatocellular carcinoma (HCC). Talin 1 (TLN1) is highly expressed in glioblastoma multiforme (GBM). The inhibition of TLN1 activity helps in diminishing malignant features of GBM cells. It also plays an important role in regulation of, Bevacizumab (anti-angiogenic agent) -resistance.
Talin 1 assists in the attachment of adherent cells to the extracellular matrix and of lymphocytes to other cells. It plays an important role in the assembly of actin filaments and in spreading and migration of different cell types, such as fibroblasts and osteoclasts.

標靶描述

Talin is a cytoskeletal protein that is found concentratedin areas of cell-substratum and cell-cell contacts. Theencoded protein plays a significant role in the assemblyof actin filaments and in spreading and migration ofvarious cell types, includ

外觀

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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YAP regulates cell mechanics by controlling focal adhesion assembly
Nardone G
Nature Communications (2017)
High expression of Talin-1 is associated with poor prognosis in patients with nasopharyngeal carcinoma.
Xu YF
BMC Cancer (2015)
Gene duplication and functional divergence during evolution of the cytoskeletal linker protein talin
Senetar MA and McCann RO
Gene, 362, 141-152 (2005)
Wonyoung Kang et al.
Oncotarget, 6(29), 27239-27251 (2015-09-05)
Glioblastoma multiforme (GBM) possesses florid angiogenesis. However, the anti-angiogenic agent, Bevacizumab, did not improve overall survival of GBM patients. For more durable anti-angiogenic treatment, we interrogated resistant mechanisms of GBM against Bevacizumab. Serial orthotopic transplantation of in vivo Bevacizumab-treated GBM
The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions?a novel architectonic cell-cell junction system
Domke LM and Franke WW
Cell and Tissue Research, 375(2), 451-482 (2019)

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