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生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~45 kDa
物種活性
mouse (predicted), human
技術
immunoprecipitation (IP): 2-4 μg using extracts of HEK-293T cells expressing human Sirt7
indirect immunofluorescence: 2-4 μg/mL using human HEK-293T cells
western blot: 1-2 μg/mL using whole extracts of HEK-293T cells expressing human Sirt7
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... SIRT7(51547)
mouse ... Sirt7(209011)
一般說明
Nicotinamide adenine dinucleotide (NAD)-dependent histone deacetylase (Sir2) is one of the silent information regulator genes in yeast. It belongs to a family of proteins, that is found in organisms ranging from bacteria to complex eukaryotes. Proteins of this family share a core domain showing 25-60% sequence identity. The mammalian Sir2 gene family is comprised of seven members which are designated as Sirt1-7.
免疫原
synthetic peptide corresponding to amino acids 35-51 of human Sirt7, conjugated to KLH via a C-terminal cysteine residue. The sequence is identical in mouse.
應用
Anti-Sirt7 antibody produced in rabbit has been used in western blotting analysis and immunofluorescence detection.
生化／生理作用
Sirt7 (sirtuin 7) is extensively found in protein associated with active rRNA genes (rDNA), in the nucleolus. It interacts with RNA polymerase I (Pol I) and histones. Overexpression of Sirt7 increases Pol I-mediated transcription, whereas knockdown of Sirt7 or inhibition of its catalytic activity results in decreased association of Pol I with rDNA and reduces Pol I transcription. Depletion of Sirt7 stops cell proliferation and triggers apoptosis. High levels of Sirt7 expression are associated with breast cancer.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression, and chromosome stability.
Brachmann CB, et al.
Genes & Development, 9(23), 2888-2902 (1995)
Involvement of SIRT7 in resumption of rDNA transcription at the exit from mitosis
Grob A, et al.
Journal of Cell Science, 122(4), 489-498 (2009)
Altered sirtuin expression is associated with node-positive breast cancer
Ashraf N, et al.
British Journal of Cancer, 95(8), 1056-1056 (2006)
Shashi Kiran et al.
The FEBS journal, 280(14), 3451-3466 (2013-05-18)
Sirtuins belong to a class of NAD-dependent deacetylases, and include seven distinct isoforms, of which SIRT7 is the least studied member. In the present study, the subcellular expression of SIRT7 in primary fibroblasts undergoing senescence was evaluated by immunocytochemistry and
The human silent information regulator (Sir) 2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
Schwer B, et al.
The Journal of Cell Biology, 158(4), 647-657 (2002)
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