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Merck

S1577

Sigma-Aldrich

Stromal Cell-Derived Factor 1α/pre-B Cell Growth Stimulating Factor human

recombinant, expressed in E. coli, lyophilized powder, ≥97% (SDS-PAGE), suitable for cell culture

别名:

SDF-1, SDF1, SDF-1α

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

human

品質等級

重組細胞

expressed in E. coli

化驗

≥97% (SDS-PAGE)

形狀

lyophilized powder

效力

≤50 ng/mL EC50

包裝

pkg of 10 μg

儲存條件

avoid repeated freeze/thaw cycles

技術

cell culture | mammalian: suitable

雜質

≤1 EU/μg protein Endotoxin

顏色

white

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... CXCL12((6387)

一般說明

Stromal Cell-Derived Factor 1α (SDF-1α) or pre-B Cell Growth Stimulation Factor (PBSF) is an 8 kDa chemokine peptide. SDF-1 was identified using signal sequence trap cloning. With this method, cDNAs have been cloned using mouse and human stromal cell lines. This cytokine is widely accepted as a CXC chemokine of the intercrine-macrophage inflammatory protein superfamily. The gene has been mapped to chromosome 10q and is encoded by 4 exons. Expression of the human SDF1 gene is abundant in the pancreas, spleen, ovary, and small intestine. The presence of a GC-rich sequence and the lack of a TATA box in the 5∅-flanking region of the gene are consistent with ubiquitous expression.

生化/生理作用

SDF-1α functions as a pre-B cell growth factor in the presence of IL-7 (interleukin-7) and a potent chemoattractant for T-lymphocytes and monocytes. It has also been found to be a ligand for CXCR4 (chemokine receptor type 4) or the orphan receptor LESTR/fusin (putative chemokine receptor). By signaling through the receptor, SDF1 may serve as an inhibitor of HIV-1 (human immunodeficiency virus), which utilizes the LESTR/fusin receptor as a point of entry. The SDF-1/CXCR4 signalling pathway is associated with cancer cell proliferation, migration and survival. Overexpression of SDF1 is observed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 offers CXCR4 mediated neuroprotection during post ischemic stroke.

外觀

This product is lyophilized from a 0.2 μm-filtered solution containing 10 μg SDF-1α and 0.5 mg bovine serum albumin in PBS.

重構

Reconstitute the contents of the vial using sterile buffer or medium containing a minimum of 0.1% BSA or HSA to a stock concentration of ≥10 μg/ml. Additional filtration of the stock solution is not recommended as this may result in loss of product due to adsorption onto the filter membrane.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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访问文档库

Diversity and inter-connections in the CXCR4 chemokine receptor/ligand family: molecular perspectives
Pawig L, et al.
Frontiers in Immunology (2015)
Structure and Chromosomal Localization of the Human Stromal Cell-Derived Factor 1 (SDF1) Gene
Shirozu M, et al.
Genomics, 28, 495-500 (1995)
SDF-1/CXCR4 expression is an independent negative prognostic biomarker in patients with head and neck cancer after primary radiochemotherapy
Colle CD, et al.
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology, 126, 125-131 (2018)
The α-Chemokine, Stromal Cell-derived Factor-1α, Binds to the
Transmembrane G-protein-coupled CXCR-4 Receptor and
Activates Multiple Signal Transduction Pathways.
Ganju RK, et al.
The Journal of Biological Chemistry, 273, 23169?- 233175 (1998)
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
International Journal of Clinical and Experimental Pathology, 8(10), 12357?12367-12357?12367 (2015)

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