推荐产品
生物源
bovine pancreas
產品線
BioReagent
化驗
≥80% (SDS-PAGE)
形狀
powder
比活性
≥50 Kunitz units/mg protein
濃度
≥60%
技術
cell based assay: suitable
適合性
suitable for molecular biology
應用
cell analysis
異物活動
protease ≤0.001 units/mg solid
儲存溫度
−20°C
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI 密鑰
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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應用
在细胞周期平台分析期间，牛胰腺核糖核酸酶B可用于消化RNA。
生化／生理作用
通过枯草杆菌蛋白酶消化天然RNase B（包含RNase B的氨基酸残基21-124）产生的天然RNase BS对PNGase F消化敏感。 牛胰腺RNase B的分子内N-聚糖具有伴侣蛋白的作用。 已有研究发现RNase B比RNase A起效更快，而RNase A在再生过程中易于聚集。Asn-寡糖（对应于RNase B的最主要糖链）的刺激作用表面RNase B的N-聚糖可促进大体积中间体转化为折叠的紧密物种。
包裝
包装尺寸取决于蛋白质含量
準備報告
通过亲和层析纯化
抑制劑
产品编号
说明
价格
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Véronique Blanchard et al.
Biochemistry, 47(11), 3435-3446 (2008-02-26)
In glycoanalysis protocols, N-glycans from glycoproteins are most frequently released with peptide- N (4)-( N-acetyl-beta-glucosaminyl)asparagine amidase F (PNGase F). As the enzyme is an amidase, it cleaves the NH-CO linkage between the Asn side chain and the Asn-bound GlcNAc residue.
H Yamaguchi et al.
Journal of biochemistry, 120(3), 474-477 (1996-09-01)
This paper describes a chaperone-like function of the intramolecular N-glycans of bovine pancreatic RNase B. We studied air-oxidative regeneration from reductively denatured species of RNase B and its nonglycosylated form, RNase A. RNase B was reactivated much faster than RNase
Audra A Hargett et al.
Molecules (Basel, Switzerland), 26(14) (2021-07-25)
Protein glycosylation is important in many organisms for proper protein folding, signaling, cell adhesion, protein-protein interactions, and immune responses. Thus, effectively determining the extent of glycosylation in glycoprotein therapeutics is crucial. Up to now, characterizing protein glycosylation has been carried
Vijay Ramakrishnan et al.
American journal of hematology, 85(9), 675-686 (2010-07-24)
Interaction of myeloma cells with the bone marrow microenvironment is mediated in large part through different cytokines, especially VEGF and IL6. These cytokines, especially IL6, leads to upregulation of the JAK/STAT pathway in myeloma cell, contributing to increased proliferation, decreased
Yang Xu et al.
iScience, 25(8), 104753-104753 (2022-08-10)
N-Acetylglucosamine (GlcNAc) is an essential monosaccharide required in almost all organisms. Fluorescent labeling of the peptidoglycan (PG) on N-acetylglucosamine has been poorly explored. Here, we report on the labeling of the PG with a bioorthogonal handle on the GlcNAc. We
商品
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
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