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一般說明
核糖核酸酶抑制剂通过形成紧密非共价的1:1复合物抑制RNase活性。 来源于人胎盘。 抑制RNA酶A、B和C。不抑制RNA酶H、1、T1、S1核酸酶、SP6、T7 RNA聚合酶、T3 RNA聚合酶、AMV逆转录酶、M-MLV逆转录酶或Taq聚合酶。 抑制剂可通过苯酚萃取或加热至65℃ 10分钟去除。
應用
用于体外抑制核糖核酸酶，包括cDNA合成、RT-PCR、及体外转录和翻译等实验。
适用于
- 体外抑制核糖核酸酶
- cDNA合成
- RT-PCR
- 体外转录和翻译
生化／生理作用
通过形成紧密非共价的1:1复合体抑制RNase活性。也可抑制血管生成素，一种与RNase A同源的新生血管促进因子。
成分
核糖核酸酶抑制剂溶液，含20 mM HEPES-KOH( pH 7.6)、50 mM KCl、8 mM DTT和50%甘油。
其他說明
这种RNA酶活性抑制剂分离自人类胎盘，为甘油缓冲水溶液形式。
溶液，pH 7.6，含50%甘油、20 mM HEPES-KOH、50 mM KCl和8 mM DTT
單位定義
一个单位的抑制剂可将一个胞苷2′:3′-环单磷酸盐体系中5 ng核糖核酸酶A的活性降低50%。
外觀
溶液，pH 7.6，含50%甘油、20 mM HEPES-KOH、50 mM KCl和8 mM DTT
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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