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重組細胞
expressed in unspecified host
品質等級
共軛
biotin conjugate
形狀
lyophilized powder
成份
Protein, ~90% biuret
標籤範圍
2-4 mol d-biotin per mol protein
技術
direct ELISA: 1:70,000 using human IgG
儲存溫度
2-8°C
一般說明
Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.
特異性
Binds IgG only from most mammalian species except cat; binds chicken IgG.
應用
Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.
包裝
Package size based on protein content
其他說明
Truncated - lacks albumin, Fab and membrane binding sites.
準備報告
Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Electrochemical biosensing based on universal affinity biocomposite platforms
Zacco E, et al.
Biosensors And Bioelectronics, 21(7), 1291-1301 (2006)
Ayumi Taniguchi et al.
Journal of nutritional science and vitaminology, 53(6), 457-463 (2008-01-19)
It is well known that biotin deficiency causes morphological anomalies in hatchlings of fowl. An abundance of biotin in the yolk, therefore, is greatly required for maintaining reproductive functions. Although there is growing evidence for the molecular significance of the
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems: Biosensors, Recognition Receptors, and Microsystem, 21(7), 360-360 (2008)
Pierre-Henri Puech et al.
PloS one, 6(7), e22344-e22344 (2011-07-30)
The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends
Alina Sultanova et al.
Microbiology spectrum, 10(3), e0236921-e0236921 (2022-05-24)
Human herpesvirus-6 (HHV-6) contains two genes (U12 and U51) that encode putative homologues of human G-protein-coupled receptors like CCR1, CCR3, and CCR5. It has been shown that these viral proteins can be expressed on the surface of epithelial and some
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