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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
5D6, monoclonal
形狀
buffered aqueous solution
物種活性
rat
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using cytosolic and membrane extracts of rat liver
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
rat ... Uso1(56042)
一般說明
Monoclonal Anti-p115/TAP (mouse IgG1 isotype) is derived from the 5D6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice. p115, a Golgin family tether is also referred as TAP1 (transcytosis associated protein). It is homologous to USO1 vesicle transport factor (Uso1p). It has a coiled coil dimerization domain, an N-terminal globular domain and a C-terminal acidic domain.
特異性
Monoclonal Anti-p115/TAP recognizes rat p115/TAP (approx. 95-100 kDa).
免疫原
purified rat p115/TAP.
應用
Monoclonal Anti-p115/TAP antibody produced in mouse may be used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
生化／生理作用
p115/TAP1 (transcytosis associated protein) plays an important role in endoplasmic reticulum to Golgi traffic and in intra−Golgi traffic. It is known to play a key role in the transport of endoplasmic reticulum (ER)/Golgi. p115 is highly essential for the docking or tethering step in intra-Golgi vesicular transport and transcytosis. It serve as a catalyst during the fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE).
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.
免責聲明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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On and off membrane dynamics of the endoplasmic reticulum-Golgi tethering factor p115 in vivo
Brandon E, et al.
Molecular Biology of the Cell, 17(7), 2996-3008 (2006)
M Barroso et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(2), 527-531 (1995-01-17)
Transcytosis-associated protein (TAP) is found on transytotic vesicles (TCVs) and is required for their fusion with the target membrane. We developed a cell-free assay capable of differentiating targeting/binding of TCVs to membrane from later fusion events. We found that TAP
Gabriella Frigerio et al.
Traffic (Copenhagen, Denmark), 8(11), 1644-1655 (2007-09-01)
ADP-ribosylation factors (ARFs) are critical regulators of vesicular trafficking pathways and act at multiple intracellular sites. ADP-ribosylation factor-GTPase-activating proteins (ARFGAPs) are proposed to contribute to site-specific regulation. In yeast, two distinct proteins, Glo3p and Gcs1p, together provide overlapping, essential ARFGAP
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