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Merck

P0493

Sigma-Aldrich

单克隆抗 PRMT5 小鼠抗

~2 mg/mL, clone PRMT5-21, purified immunoglobulin, buffered aqueous solution

别名:

Anti-Protein-Arginine Methyl Transferase 5

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

PRMT5-21, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~70 kDa by SDS-PAGE

物種活性

mouse, hamster, bovine, canine, monkey, rat, human, chicken

濃度

~2 mg/mL

技術

indirect ELISA: suitable
microarray: suitable
western blot: 2 μg/mL using whole cell extract of human melanoma cell line G361

同型

IgG2a

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... PRMT5(10419)

一般說明

Monoclonal Anti-PRMT5 (mouse IgG2a isotype) is derived from the PRMT5-21 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with GST-PRMT5 containing the C-terminal region of the human PRMT5. Protein arginine methyltransferases (PRMT) 5 belongs to the type II arginine methyltransferases. PRMT5 (also known as Skb1Hs/janus kinase-binding protein 1 (JBP1) exists as homo-oligomeric complexes, which includes a dimer and tetramer. PRMT5 is found mainly in the cytoplasm. This gene is located on human chromosome 14q11.2.

免疫原

GST-PRMT5 containing the C-terminal region of the human PRMT5 (amino acids 315-637).

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-PRMT5 antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

生化/生理作用

Protein arginine methyltransferase 5 (PRMT5) participates in the symmetrical dimethylation of arginine residues. Homooligomerization of PRMT5 has an important role in its ability to methylate the myelin basic protein (MBP) protein. PRMT5 also binds the Janus kinases and are involved in an interferon-signaling pathway. It also helps to produce symmetrical ω-NG-dimethylarginine.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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在文件库中查找您最近购买产品的文档。

访问文档库

LKB1 regulates PRMT5 activity in breast cancer
Lattouf H, et al.
International Journal of Cancer. Journal International Du Cancer, 144(3), 595-606 (2019)
Ming Liu et al.
Theranostics, 10(10), 4437-4452 (2020-04-16)
The proto-oncogene c-Myc regulates multiple biological processes mainly through selectively activating gene expression. However, the mechanisms underlying c-Myc-mediated gene repression in the context of cancer remain less clear. This study aimed to clarify the role of PRMT5 in the transcriptional
PRMT5 dimethylates R30 of the p65 subunit to activate NF-?B.
Wei H
Proceedings of the National Academy of Sciences of the USA, 110(33), 13516-13521 (2013)
Expression of protein arginine methyltransferase-5 in oral squamous cell carcinoma and its significance in epithelial-to-mesenchymal transition
Amano Y, et al.
Pathology international, 68(6), 359-366 (2018)
Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family
Rho J, et al.
The Journal of biological chemistry, 276(14), 11393-11401 (2001)

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