O9635

抑癌蛋白 M 人

BioReagent, ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 别名: OSM
• CAS号: 106956-32-5
• EC號碼: 200-835-2
• MDL號碼: MFCD00166731
• 分類程式碼代碼: 12352202
• NACRES: NA.32

属性

• 生物源: human
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 產品線: BioReagent
• 化驗: ≥97% (SDS-PAGE)
• 形狀: lyophilized powder
• 效力: ≤1.000 ng/mL ED₅₀
• 分子量: ~22 kDa
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 技術: cell culture | mammalian: suitable
• 雜質: endotoxin, tested
• 顏色: white
• 溶解度: soluble 0.100 mL, clear, colorless (Solvent-PBS + 0.1% BSA)
• UniProt登錄號: P13725
• 儲存溫度: −20°C
• 基因資訊: human ... OSM(5008)

说明

一般說明

Oncostatin M (OSM) is produced by activated T cells, monocytes and Kaposi′s sarcoma cells. OSM shares several structural and functional characteristics with leukemia inhibitory factor (LIF), interleukin-6 (IL-6), and ciliary neurotrophic factor (CNTF).

應用

Human oncostatin-M has been used to stimulate bovine full-depth cartilage explants. It has also been used to culture embryonic stem cells in standard minimum essential medium-α.

生化／生理作用

Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. It can exert both stimulatory and inhibitory effects on cell proliferation. OSM stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi′s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release [e.g. interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte-colony-stimulating factor (G-CSF)] from endothelial cells.

Oncostatin M (OSM), LIF, G-CSF, IL-6, and CNTF are structurally related members of the same cytokine family sharing similarities in their primary amino acid sequences, predicted secondary structure, and receptor components. OSM is a growth-regulating cytokine, affecting a number of tumor and normal cells. This material was first identified by its ability to inhibit the growth of A375 melanoma cells and other human tumor cells, but not inhibit the growth of normal human fibroblasts. It acts synergistically with TGF β1 to inhibit the proliferation of tumor cells like A375 melanoma cells. It induces an increase in LDL receptor expression and LDL uptake by hepatoma cells. OSM activates synovial fibroblast-like cells to produce urokinase type plasminogen activator. OSM is secreted by macrophages and activated T lymphocytes.

外觀

Lyophilized from a 0.2 μm filtered solution in 10 mM acetic acid, containing 50 μg of bovine serum albumin per 1 μg of cytokine.

分析報告

The proliferative activity of human oncostatin M is tested in culture by using a human erythroleukemic cell line, TF-1.

安全信息

• 儲存類別代碼: 4.1B - Flammable solid hazardous materials
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter