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Merck

N9532

Sigma-Aldrich

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

clone NOS-E1, ascites fluid

别名:

Anti-eNOS

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41
共軛:
unconjugated
application:
ARR
WB
無性繁殖:
NOS-E1, monoclonal
物種活性:
rat, mouse, human, bovine
citations:
15
技術:
microarray: suitable
western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

NOS-E1, monoclonal

分子量

antigen 135 kDa

包含

15 mM sodium azide

物種活性

rat, mouse, human, bovine

技術

microarray: suitable
western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells

同型

IgA

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)

一般說明

Monoclonal Anti-Nitric Oxide Synthase, endothelial (eNOS) (mouse IgA isotype) is derived from the NOS-E1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. NOS consists of heme and calmodulin binding sites located within its N-terminal half. It has been localized in many different cell types. Based on molecular mass, subcellular location and Ca2+ dependence, at least three types of NOS have been classified. Type I NOS (150-160 kD protein) is found in neurons and is also called as neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca2+-regulated NOS (cNOS). Type II (130 kD protein) is found in macrophages and is also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kD protein) is found in endothelial cells and is also called endothelial NOS (eNOS or ecNOS).
The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.

免疫原

synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.

應用

Monoclonal Anti-Nitric Oxide Synthase, Endothelial (eNOS) has been used:
  • for enzyme linked immunosorbent assay (ELISA)
  • immunoblot
  • immunohistochemistry
  • immunofluorescence.

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse has been used in antibody microarray.

生化/生理作用

Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease.
NOS catalyses the oxidation of guanidino nitrogen of arginine. The released nitric oxide mediates vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. NOS is associated with multiple sclerosis and allergic encephalomyelitis.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析证书(COA)

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访问文档库

Beth A McNichol et al.
Infection and immunity, 75(11), 5095-5104 (2007-08-22)
Cytotoxic necrotizing factor type 1 (CNF1) and CNF2 are highly homologous toxins that are produced by certain pathogenic strains of Escherichia coli. These 1,014-amino-acid toxins catalyze the deamidation of a specific glutamine residue in RhoA, Rac1, and Cdc42 and consist
Post-Transcriptional Regulation of Endothelial Nitric Oxide Synthase Expression by Polypyrimidine Tract-Binding Protein 1.
Yi B
Arteriosclerosis and Thrombosis (2015)
Synthesis and biological evaluation of novel 1, 5-benzothiazepin-4 (5H)-ones as potent antiangiogenic and antioxidant agents
Deepu C, et al.
Current Chemistry Letters, 4(4), 133-144 (2015)
Association between three eNOS polymorphisms and intracranial aneurysms risk: a meta-analysis.
Yang C
Medicine (2015)
Surgical Research, 951-951 (2001)

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