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Merck

N8786

Sigma-Aldrich

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal

clone 414, purified from hybridoma cell culture

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

414, monoclonal

形狀

buffered aqueous solution

分子量

antigen 62-210 kDa

物種活性

mouse, rat, yeast, Xenopus, human

濃度

~3 mg/mL

技術

electron microscopy: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using HeLa total cell extract

同型

IgG1

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

一般說明

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 414 produced by the fusion of mouse myeloma cells (NS-1 cells) and splenocytes from BALB/c mice immunized with rat liver nuclei extract. The nuclear pore complexes (NPCs) are structurally highly conserved in all eukaryotic cells. They contain 30-40 different proteins resulting in a complex with a molecular weight of ~50 kDa.
Nuclear pore complexes (NPCs) are aqueous channels that impart access to the nucleus and modulate the transport of RNA, proteins and ribosome subunits across the nuclear envelope. Nucleoporins, which are conserved proteins, form a complex network and generate these channels. Each NPC is a large, complex cylindrical structure consisting of around 500 nucleoporins.

免疫原

rat liver nuclei extract.

應用

Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used for immunohistochemistry.
Anti-Nuclear Pore Complex Proteins antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunoelectron microscopy
  • immunofluorescence

生化/生理作用

Nucleocytoplasmic exchanges occur through nuclear pore complexes (NPCs) that are circular apertures in the nuclear envelope where the inner and the outer nuclear membrane are joined. Through the NPC there is a passive transport of small molecules (ions and water) that are smaller than 9-10 nm. Large molecules such as RNA and proteins have to be actively transported through the NPC in an energy and signal dependent manner. Many proteins interact with members of the NPC complex.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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在文件库中查找您最近购买产品的文档。

访问文档库

The Nuclear Pore Complex and Nuclear Transport
Susan R
Cold Spring Harbor Perspectives in Biology (2010)
Satya Prakash Singh et al.
Biochemical and biophysical research communications, 511(1), 192-198 (2019-02-20)
Vpx, a virion-associated protein of Human Immunodeficiency Virus 2 (HIV-2) and Simian Immunodeficiency Virus (SIV) counteracts host restriction factor SAMDH1 for efficient viral DNA synthesis in the cytoplasm and mediates subsequent nuclear translocation of the viral genome. Vpx was found
Lentiviral Vpx induces alteration of mammalian cell nuclear envelope integrity
Singh SP, et al.
Biochemical and Biophysical Research Communications, 511(1) (2019)
EGF Signaling and the Origin of Axial Polarity among the Insects
Jeremy A
Current Biology (2010)
Lentiviral Vpx induces alteration of mammalian cell nuclear envelope integrity
Singh SP, et al.
Biochemical and biophysical research communications, 511(1) (2019)

商品

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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