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MTOX1011

Sigma-Aldrich

PXR Knockout HepaRG™ Cells

1 vial

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.81

生物源

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

品質等級

200

形狀

liquid

OMIM登錄號

603065

儲存溫度

−196°C

基因資訊

human ... NR1I2(1337040)

一般說明

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG PXR KO cells express the major xenobiotic sensors (CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

應用

See technical bulletin for detailed protocols

特點和優勢

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

  • Sigma′s HepaRG PXR Knockout (KO) allows conclusive identification of CYP3A4 induction through PXR activation.

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I2 (PXR) gene.

  • The frame-shift mutation of NR1I2 gene was confirmed by fragment length analysis and DNA sequencing.
  • Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

品質

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

法律資訊

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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