推荐产品
檢測方法
colorimetric
相關疾病
immunological diseases
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
β-Lactamases are a large family of enzymes capable of hydrolyzing β-lactams. A β-Lactam ring is the common element in all beta-lactam antibiotics including penicillin derivatives, cephalosporins, monobactams, and carbapenems. Through hydrolysis, β-lactamase breaks the β-lactam ring open, deactivating the antibacterial properties. Bacteria from clinical and non-clinical settings are becoming increasingly resistant to β-lactam antibiotics by synthesizing β-lactamase. To overcome this resistance, β-lactam antibiotics are often given with β-lactamase inhibitors such as clavulanic acid. Therefore, detection of β-lactamase activity is of central importance to assess beta-lactam antibiotics as well as to prevent antibiotics resistance.
The Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm.
The Beta-Lactamase Activity Assay Kit offers a sensitive colorimetric assay for measuring β-lactamase activity in biological samples. The assay can be performed using an absorbance microplate reader by measuring the OD ratio at the wavelength of 490 nm to 380 nm.
原則
The β-lactamase activity is detected using nitrocefin, which changes color from yellow to red upon hydrolysis by β-lactamase.
One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 µmole of nitrocefin per minute at pH 7.0 at 25°C.
One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 µmole of nitrocefin per minute at pH 7.0 at 25°C.
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
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