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用途
sufficient for 100 colorimetric tests
檢測方法
colorimetric
相關疾病
neurological disorders; cardiovascular diseases; cancer
儲存溫度
2-8°C
基因資訊
human ... ACO1(48) , ACO2(50)
mouse ... ACO1(11428) , ACO2(11429)
rat ... ACO1(50655) , ACO2(79250)
一般說明
该乌头酸酶是一个含铁-硫的酶家族，其催化柠檬酸异构化为异柠檬酸。人们已经在真核生物中鉴定出了由不同基因编码的两种乌头酸酶。乌头酸酶 1（ACO1，c-乌头酸酶）是一种胞质酶，而乌头酸酶 2（ACO2，m-乌头酸酶）是一种在三羧酸循环中起作用的线粒体酶。乌头碱可被氧化应激可逆地失活，并且细胞和组织中的乌头酸酶活性已被用作一种氧化损伤的生物标志物。
應用
乌头酸酶活性检测试剂盒已被用于测量细胞溶质和线粒体顺乌头酸酶的活性。
特點和優勢
兼容高通量处理系统。
適合性
适用于测量组织和细胞等生物样品中的乌头酸酶活性
原則
乌头酸酶活性检测试剂盒提供了一种测量各种样品中的乌头酸酶活性的简单、直接的方法。在偶联酶反应中测定乌头酸酶活性，在该反应中柠檬酸盐被乌头酸酶转化为了异柠檬酸。因此，比色（450nm）产物与酶活性的量成正比。一个单位的乌头酸酶是指在 25℃，pH 7.4条件下每分钟将异柠檬酸异构化产生 1.0 μmole 柠檬酸盐的酶量。
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
儲存類別代碼
8A - Combustible corrosive hazardous materials
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