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生物源
equine skeletal muscle
產品線
BioUltra
化驗
95-100% (SDS-PAGE)
形狀
essentially salt-free, lyophilized powder
分子量
~17 kDa(lit.)
純化經由
affinity chromatography
鐵含量含量
~0.30%
技術
mass spectrometry (MS): suitable
溶解度
H2O: soluble 10 mg/mL
UniProt登錄號
儲存溫度
−20°C
基因資訊
horse ... MB(100054434)
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應用
肌红蛋白被用作质谱和X射线晶体学分析的分子量标记物和标准品。来自马骨骼肌的肌红蛋白被用于测试实验蛋白质混合物以验证串联质谱分析。
马骨骼肌中的肌红蛋白可用于诱发小鼠急性肾损伤(AKI)。它可用作自上而下质谱分析的标准物。
生化／生理作用
来自马骨骼肌的肌红蛋白是一种含有153个氨基酸残基的单链血红素蛋白。 它不含二硫键或游离-SH基团。肌红蛋白含有8个不同大小的右旋螺旋区域，由无序或随机的卷曲区域连接。
肌红蛋白对于近最大需氧量的骨骼肌O2 供应至关重要，并且通过将PO2维持在支持线粒体功能所需水平以上来防止缺氧。
肌红蛋白对于近最大需氧量的骨骼肌O2 供应至关重要，并且通过将PO2维持在支持线粒体功能所需水平以上来防止缺氧。
肌红蛋白对骨骼肌在接近最大氧气需求时的O2 的供应至关重要，并通过将PO2 维持在高于支持线粒体功能所需的水平来防止缺氧。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Z K Shihabi
Journal of chromatography. B, Biomedical applications, 669(1), 53-58 (1995-07-07)
Capillary electrophoresis was used in this study to separate urinary myoglobin from hemoglobin based on its electrophoretic mobility. Urine was applied directly without any treatment. The separation was accomplished in less than 7 min. Myoglobin extracted from human muscle tissues
Rhabdomyolysis induced AKI via the regulation of endoplasmic reticulum stress and oxidative stress in PTECs
Feng Y, et al.
Royal Society of Chemistry Advances, 6(111), 109639-109648 (2016)
J Zaia et al.
Rapid communications in mass spectrometry : RCM, 6(1), 32-36 (1992-01-01)
Myoglobins from horse heart muscle, horse skeletal muscle and sperm whale are widely used as calibration standards or test compounds for various mass spectrometric methodologies. In all such cases reported in the literature, a molecular weight value is used (16,950.5
S C Powell et al.
Journal of chromatography, 317, 87-92 (1984-12-28)
Urine and serum myoglobin have been separated on an anion-exchange column, packed by the slurry technique. Urine or serum was injected directly into the column and eluted isocratically with Tris buffer. Freshly prepared myoglobin from human muscle gives two peaks
Protein Bioinformatics (2017)
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Local delivery of bioactive molecules using an implantable device can decrease the amount of drug dose required as well as non-target site toxicities compared to oral or systemic drug administration.
Chromatograms
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