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Merck

L8754

Sigma-Aldrich

来自 菜豆的凝集素 (红芸豆)

Phytohemagglutinin PHA-P, lyophilized powder

别名:

PHA, 植物血球凝集素(菜豆)

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About This Item

EC號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

生物源

Phaseolus vulgaris

品質等級

形狀

lyophilized powder

效力

≤62.5 μg per mL agglutination activity
≤10 μg/mL mitogenic activity

雜質

salt, free

儲存溫度

2-8°C

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相关类别

一般說明

PHA是碳水化合物结合蛋白。已鉴定出几种类型的PHA,主要存在于豆科植物中。
植物血凝素(PHA)是在植物中发现的一种凝集素(特别是在豆科植物中)。它来自于 菜豆(Phaseolus vulgaris) (红芸豆),并可作为人类白细胞培养物中的有丝分裂原。

應用

可用作抗原,测定 体内 T细胞介导的免疫应答。
来自 菜豆(Phaseolus vulgaris) (红芸豆)的凝集素可用于植物血凝素(PHA)检测 ,以及评估细胞和体液免疫力。

生化/生理作用

PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

分析報告

凝集活性以mg/ml表示,并由1mg/ml溶液的连续稀释液进行测定。该活性是在25℃温育1小时后,在pH6.8的磷酸盐缓冲盐水中,凝集2%人红细胞悬浮液所需的最低浓度。其促有丝分裂活性可通过在HeLa细胞培养物中掺入BrdU来确定。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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