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Merck
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主要文件

L8384

Sigma-Aldrich

亚油酸蛋白 来源于牛血清白蛋白

γ-irradiated, lyophilized powder, BioReagent, suitable for cell culture

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.75

生物源

bovine plasma

品質等級

描述

Contains 1.1-2.3 moles linoleic acid per mole BSA

無菌

γ-irradiated

產品線

BioReagent

形狀

lyophilized powder

濃度

>0.5% (linolenic acid)

技術

cell culture | mammalian: suitable

雜質

≤50 EU/mg endotoxin

溶解度

water: 10 mg/mL, clear to hazy, colorless to light yellow

儲存溫度

2-8°C

一般說明

Linoleic acid is an essential and highly consumed polyunsaturated fatty acid (PUFA). Albumin is a globular protein. Bovine serum albumin (BSA) is composed of a single polypeptide chain and cross-linked cysteine disulfide bridges. BSA is heart-shaped and is made up of three homologous domains I-III and each domain has two subdomains A and B.

應用

Linoleic Acid-Albumin from bovine serum albumin has been used: in cell culture and to study its effects on the human colonic adenocarcinoma cell line. It has also been used in the in vitro culture (IVC) of cow embryos/zygotes. The binding of linoleic acid to BSA improves its stability and solubility in culture media.

生化/生理作用

Linoleic acid plays a role in maintaining the transdermal water barrier in epidermis. Serum albumin constitutes about 52-62% of the total water-soluble fraction proteins in the blood plasma.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Takemasa Hidaka et al.
Theriogenology, 113, 176-182 (2018-03-20)
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate

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Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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